Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:334252
Zapata G, et al., Generation of a mouse model of the neurodevelopmental disorder with dysmorphic facies and distal limb anomalies syndrome. Hum Mol Genet. 2022 Oct 10;31(20):3405-3421
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7595948
|
|
Gene symbol:
|
Eomes
|
|
Gene name:
|
eomesodermin
|
|
|
Results
|
Specimen
2C Ctrl:
embryonic day 15.5
(more  )
Note: Double labeled: green - Eomes; red - EdU.
Specimen
2C Ctrl:
(close  )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Eomes; red - EdU.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
neural progenitor cell |
Present |
Regionally restricted |
2C Ctrl Merge
,
2C Ctrl Tbr2
|
Expression in intermediate progenitor cells.
|
Specimen
Het not shown:
embryonic day 15.5; Bptftm1.1Cwu/Bptf +
(more )
Specimen
Het not shown:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
|
Mutant Allele(s):
|
Bptftm1.1Cwu/Bptf+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
neural progenitor cell |
Present |
Regionally restricted |
|
Expression in intermediate progenitor cells.
|
Specimen
2C cKO:
embryonic day 15.5
(more )
Note: Double labeled: green - Eomes; red - EdU.
Specimen
2C cKO:
(close )
|
Genetic Background:
|
involves: C57BL/6
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Eomes; red - EdU.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
neural progenitor cell |
Present |
Regionally restricted |
2C cKO Tbr2
,
2C cKO Merge
|
Slight difference in overall number of cells compared to control and heterozygote.
|
|
Analysis Tools