Gene Expression Data
Assay Details
Assay
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Reference:
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J:209053
Hoekstra EJ, et al., Lmx1a is an activator of Rgs4 and Grb10 and is responsible for the correct specification of rostral and medial mdDA neurons. Eur J Neurosci. 2013 Jan;37(1):23-32
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7595755
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Gene symbol:
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Wsb1
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Gene name:
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WD repeat and SOCS box-containing 1
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Probe:
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Wsb1 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2E:
embryonic day 14.5
(more )
Specimen
2E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS22: brain |
Strong |
Widespread |
2E
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Expressed in the entire brain at high levels, and was more highly expressed in the intermediate zone.
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Specimen
2W:
embryonic day 14.5
(more )
Note: Double labeled: purple - Wsb1; brown - Th (assay MGI:7595776).
Specimen
2W:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Wsb1; brown - Th (assay MGI:7595776).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: brain |
Strong |
Widespread |
2W
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Expressed in the entire brain at high levels, and was more highly expressed in the intermediate zone. Co-localised with TH, although not with a specific expression pattern.
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Specimen
2BB:
embryonic day 14.5
(more )
Note: Double labeled: purple - Wsb1; brown - Th (assay MGI:7595776).
Specimen
2BB:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Wsb1; brown - Th (assay MGI:7595776).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: brain |
Present |
Widespread |
2BB
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Expressed in the entire brain at high levels, and was more highly expressed in the intermediate zone. Co-localised with TH, although not with a specific expression pattern.
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Specimen
3D:
embryonic day 14.5
(more )
Note: Double labeled: purple - Wsb1; brown - Th (assay MGI:7595776).
Specimen
3D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Wsb1; brown - Th (assay MGI:7595776).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
Strong |
Regionally restricted |
3D
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High levels of expression in the ventricular and intermediate zone. Co-localised with TH and Lmx1a in the mesodiencephalic dopamine progenitor area.
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TS22: midbrain ventricular layer |
Strong |
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3D
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Specimen
3M:
embryonic day 14.5
(more )
Note: Double labeled: purple - Wsb1; brown - Th (assay MGI:7595776).
Specimen
3M:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
|
Note:
|
Double labeled: purple - Wsb1; brown - Th (assay MGI:7595776).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
Strong |
Regionally restricted |
3M
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High levels of expression in the ventricular and intermediate zone. Co-localised with TH and Lmx1a in the mesodiencephalic dopamine progenitor area.
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TS22: midbrain ventricular layer |
Strong |
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3M
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Specimen
3V:
embryonic day 14.5
(more )
Note: Double labeled: purple - Wsb1; brown - Th (assay MGI:7595776).
Specimen
3V:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
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Embedding:
|
Cryosection
|
Note:
|
Double labeled: purple - Wsb1; brown - Th (assay MGI:7595776).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
Strong |
Regionally restricted |
3V
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High levels of expression in the ventricular and intermediate zone. Co-localised with TH and Lmx1a in the mesodiencephalic dopamine progenitor area.
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TS22: midbrain ventricular layer |
Strong |
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3V
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