Tg(Myh6-mCherry,-Trpv4)1Td
Transgene Detail
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| Symbol: |
Tg(Myh6-mCherry,-Trpv4)1Td |
| Name: |
transgene insertion 1, Timothy Domeier |
| MGI ID: |
MGI:7593922 |
| Transgene: |
Tg(Myh6-mCherry,-Trpv4)1Td Location: unknown
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| Alliance: |
Tg(Myh6-mCherry,-Trpv4)1Td page
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| Strain of Origin: |
Not Applicable
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| Transgene Type: |
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Transgenic (Conditional ready, Inserted expressed sequence, Reporter) |
| Mutation: |
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Insertion
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Mutation details: The Tg(alpha-MHC-loxP-mCherrySTOP-loxP-TRPV4)1td transgenic construct was created using cDNA of mouse Trpv4, the pmCherryC1 vector, the pJFRC172 vector, and the vector containing the alpha-myosin heavy chain promoter. First, pmCherryC1 was digested using BglII-BamHI for removal of the multiple cloning site, followed by AgeI-MluI (with Klenow fragment) to excise the mCherry coding region and stop codons. The mCherrySTOP sequence was then subcloned into pJFRC172 (AgeI -SpeI with Klenow fragment) resulting in a floxed mCherrySTOP sequence. The loxP-mCherrySTOP-loxP coding sequence was amplified with PCR primers encoding SalI (forward) and MluI and SalI (reverse) restriction sites, and subcloned into the SalI site of the alpha-MHC vector to form alpha-MHC-loxP-mCherrySTOP-loxP. The Trpv4 coding sequence was then amplified from cDNA with forward and reverse primers encoding BssHII restriction sites, and was inserted into the MluI site of alpha-MHC-loxP-mCherrySTOP-loxP to form alpha-MHC-loxP-mCherrySTOP-loxP-TRPV4.
(J:268386)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:268386 Thorneloe KS, et al., N-((1S)-1-{[4-((2S)-2-{[(2,4-dichlorophenyl)sulfonyl]amino}-3-hydroxypropanoyl)-1 -piperazinyl]carbonyl}-3-methylbutyl)-1-benzothiophene-2-carboxamide (GSK1016790A), a novel and potent transient receptor potential vanilloid 4 channel agonist induces urinary bladder contraction and hyperactivity: Part I. J Pharmacol Exp Ther. 2008 Aug;326(2):432-42 |
| All: |
1 reference(s) |
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