Gene Expression Data
Assay Details
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Assay
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Reference:
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J:220849
Achilleos A, et al., MBTPS1/SKI-1/S1P proprotein convertase is required for ECM signaling and axial elongation during somitogenesis and vertebral development. Hum Mol Genet. 2015 May 15;24(10):2884-98
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7593916
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Gene symbol:
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Tubb3
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Gene name:
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tubulin, beta 3 class III
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Antibody:
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anti-TuJ1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 350
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Results
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Specimen
3J:
embryonic day 11.5
(more  )
Specimen
3J:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb nerve |
Present |
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3J
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Expression in neuronal projections from spinal cord into limb.
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| TS19: spinal nerve |
Present |
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3J
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Expression in neuronal projections from spinal cord into limb.
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Specimen
3K:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * CD-1
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Mutant Allele(s):
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Mbtps1tm1Jdh/Mbtps1tm1Jdh
Tg(Col1a1-cre)1Bek/0
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: nerve |
Ambiguous |
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3K
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Authors report a general lack of neuronal projections compared to control.
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| TS19: limb nerve |
Absent |
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3K
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No neuronal projections in hind limb buds.
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Analysis Tools
