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Gene Expression Data
Assay Details
Assay
Reference: J:151457 Nelson BR, et al., Acheate-scute like 1 (Ascl1) is required for normal delta-like (Dll) gene expression and notch signaling during retinal development. Dev Dyn. 2009 Feb 2;238(9):2163-2178
Assay type: RNA in situ
MGI Accession ID: MGI:7593873
Gene symbol: Dll3
Gene name: delta like canonical Notch ligand 3
Probe: MGC clone 62323
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 1D: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: neural retina Weak Single cells 1D Weaker expression was detected in single cells in the neurogenic region of the neural retina.
Specimen 1E: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: neural retina Strong Regionally restricted 1E Strong expression was detected in the neurogenic zone.
Specimen 1F: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: neural retina Strong Regionally restricted 1F Strong expression was detected in the neurogenic zone.
Specimen 2C: embryonic day 14.5 (more )
Note: Double labeled: black - Dll3; red - Tubb3 (assay MGI:7593765).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 2C center , 2C left Dll3 expression in the neural retina doesn't co-label Tubb3-positive neurons in the retinal ganglion cell layer, nor in Tubb3-positive newborn neurons in the progenitor zone.
Specimen 2F: embryonic day 14.5 (more )
Note: Double labeled: black - Dll3; green - BrdU.
Structure Cell Type Level Pattern Image Note
TS22: neural retina retinal progenitor cell Present 2F center , 2F left Dll3-expressing cells can incorporate BrdU.
Specimen 3B: embryonic day 17.5 (more )
Note: Double labeled: black - Dll3; red - PH3.
Structure Level Pattern Image Note
TS25: neural retina Present Regionally restricted 3B middle , 3B top The majority of PH3+ mitotic progenitors at the apical surface do not co-express Dll3.
Specimen 3E: embryonic day 18.5 (more )
Note: Double labeled: black - Dll3; red - Pcna (assay MGI:7593766).
Structure Level Pattern Image Note
TS26: retina nuclear layer Present 3E center left , 3E left top , 3E left middle , 3E center right Dll3 expression overlaps with PCNA immunolabeling in the neuroblast layer. Occasional Dll3+ cells that were not PCNA+ were observed.
TS26: retina ganglion cell layer Absent 3E left middle , 3E left top , 3E center left , 3E center right
Specimen 5B: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: retina Present 5B
Specimen 5B': postnatal day 0; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS27: retina Weak 5B' Expression levels were decreased in the Ascl1-/- retina compared with normal expression levels in the Ascl1+/+ retina.
Specimen S1B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex ventricular layer Present S1B
TS20: diencephalon Strong Regionally restricted S1B Expression was more strongly expressed in the dorsal and ventral regions.
Specimen S1B inset: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted S1B inset Expression was detected in the rostral spinal cord.
Specimen S2B: embryonic day 17.5 (more )
Note: Double labeled: black - Dll3; green - BrdU.
Structure Cell Type Level Pattern Image Note
TS25: neural retina retinal progenitor cell Present S2B left , S2B center Dll3-expressing cells can incorporate BrdU.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory