Probe:	MGC clone 28345
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: neural retina	Strong	Single cells	1G	Strong expression was detected in single cells in the neurogenic region of the neural retina.
TS20: eye vasculature	Present		1G

Structure	Level	Pattern	Image	Note
TS22: neural retina	Strong	Regionally restricted	1H	Strong expression was detected in the neurogenic zone.

Structure	Level	Pattern	Image	Note
TS25: neural retina	Strong	Regionally restricted	1I	Strong expression was detected in the neurogenic zone.

Structure	Level	Pattern	Image	Note
TS22: neural retina	Present	Regionally restricted	2D left , 2D center	Dll4 expression in the neural retina doesn't co-label Tubb3-positive neurons in the retinal ganglion cell layer, nor in Tubb3-positive newborn neurons in the progenitor zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: neural retina	retinal progenitor cell	Present		2G center , 2G left	Dll4-expressing cells can incorporate BrdU.

Structure	Level	Pattern	Image	Note
TS25: neural retina	Present	Regionally restricted	3C top , 3C middle	The majority of PH3+ mitotic progenitors at the apical surface do not co-express Dll4.

Structure	Level	Pattern	Image	Note
TS26: retina nuclear layer	Present		3F left top , 3F center left , 3F center right , 3F left middle	Dll4 expression overlaps with PCNA immunolabeling in the neuroblast layer.
TS26: retina ganglion cell layer	Absent		3F left top , 3F center right , 3F center left , 3F left middle

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Absent		S1C
TS20: diencephalon	Absent		S1C

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	S1C inset	Expression was detected in a small region in the middle of the rostral spinal cord.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: neural retina	retinal progenitor cell	Present		S2C center , S2C left	Dll4-expressing cells can incorporate BrdU.