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Gene Expression Data
Assay Details
Assay
Reference: J:330213 Ebeid M, et al., PRDM16 expression and function in mammalian cochlear development. Dev Dyn. 2022 Oct;251(10):1666-1683
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7581970
Gene symbol: Prdm16
Gene name: PR domain containing 16
Antibody: Anti-Prdm16
Results
Specimen 1B E13.5: embryonic day 13.5 (more )
Note: Double labeled: green - Prdm16; red - Sox2 (assay MGI:7581990).
Structure Cell Type Level Pattern Image Note
TS21: greater epithelial ridge Present Regionally restricted 1B E13.5 Expression was detected within the nuclei of Kolliker's organ cells. Expression appeared first in the basal and middle regions of the cochlear duct.
TS21: cochlea supporting cell of cochlea Present Regionally restricted 1B E13.5 Partial overlap of expression with Sox2 was noted in the region of inner supporting cells.
Specimen 1B E14.5: embryonic day 14.5 (more )
Note: Double labeled: green - Prdm16; red - Sox2 (assay MGI:7581990).
Structure Cell Type Level Pattern Image Note
TS22: greater epithelial ridge Present Regionally restricted 1B E14.5 Expression was detected within the nuclei of Kolliker's organ cells. Expression appeared first in the basal and middle regions of the cochlear duct.
TS22: cochlea supporting cell of cochlea Present Regionally restricted 1B E14.5 Partial overlap of expression with Sox2 was noted in the region of inner supporting cells.
Specimen 1B E15.5: embryonic day 15.5 (more )
Note: Double labeled: green - Prdm16; red - Sox2 (assay MGI:7581990).
Structure Cell Type Level Pattern Image Note
TS23: greater epithelial ridge Present Regionally restricted 1B E15.5 Expression was detected within the nuclei of Kolliker's organ cells. Expression was in the apical, basal and middle regions of the cochlear duct.
TS23: cochlea supporting cell of cochlea Present Regionally restricted 1B E15.5 Partial overlap of expression with Sox2 was noted in the region of inner supporting cells.
Specimen 1B E16.5: embryonic day 16.5 (more )
Note: Double labeled: green - Prdm16; red - Sox2 (assay MGI:7581990).
Structure Cell Type Level Pattern Image Note
TS24: greater epithelial ridge Present Regionally restricted 1B E16.5 Expression was detected within the nuclei of Kolliker's organ cells. Expression was in the apical, basal and middle regions of the cochlear duct.
TS24: cochlea supporting cell of cochlea Present Regionally restricted 1B E16.5 Partial overlap of expression with Sox2 was noted in the region of inner supporting cells.
TS24: cochlea interdental cell of cochlea Moderate 1B E16.5
TS24: cochlear duct Weak Regionally restricted 1B E16.5 Weak expression was detected in the stria vascularis.
Specimen 1B E18.5: embryonic day 18.5 (more )
Note: Double labeled: green - Prdm16; red - Sox2 (assay MGI:7581990).
Structure Cell Type Level Pattern Image Note
TS26: greater epithelial ridge Present Regionally restricted 1B E18.5 Expression was detected within the nuclei of Kolliker's organ cells. Expression was in the apical, basal and middle regions of the cochlear duct.
TS26: cochlea Present Regionally restricted 1B E18.5 Partial overlap of expression with Sox2 was noted in the region of inner supporting cells.
TS26: cochlea interdental cell of cochlea Moderate 1B E18.5
TS26: stria vascularis Weak 1B E18.5
Specimen 1B P0: postnatal day 0 (more )
Note: Double labeled: green - Prdm16; red - Sox2 (assay MGI:7581990).
Structure Cell Type Level Pattern Image Note
TS27: greater epithelial ridge Present Regionally restricted 1B P0 Expression was detected within the nuclei of Kolliker's organ cells. Expression was in the apical, basal and middle regions of the cochlear duct.
TS27: cochlea supporting cell of cochlea Present Regionally restricted 1B P0 Partial overlap of expression with Sox2 was noted in the region of inner supporting cells.
TS27: cochlea interdental cell of cochlea Moderate 1B P0
TS27: stria vascularis Weak 1B P0
Specimen 1B P3: postnatal day 3 (more )
Note: Double labeled: green - Prdm16; red - Sox2 (assay MGI:7581990).
Structure Cell Type Level Pattern Image Note
TS27: greater epithelial ridge Present Regionally restricted 1B P3 Expression was detected within the nuclei of Kolliker's organ cells. Expression was in the apical, basal and middle regions of the cochlear duct.
TS27: cochlea supporting cell of cochlea Present Regionally restricted 1B P3 Partial overlap of expression with Sox2 was noted in the region of inner supporting cells.
TS27: cochlea interdental cell of cochlea Moderate 1B P3
TS27: stria vascularis Weak 1B P3
Specimen 1B P7: postnatal day 7 (more )
Note: Double labeled: green - Prdm16; red - Sox2 (assay MGI:7581990).
Structure Cell Type Level Pattern Image Note
TS28: greater epithelial ridge Present Regionally restricted 1B P7 Expression was detected within the nuclei of Kolliker's organ cells. Expression was in the apical, basal and middle regions of the cochlear duct.
TS28: cochlea supporting cell of cochlea Present Regionally restricted 1B P7 Partial overlap of expression with Sox2 was noted in the region of inner supporting cells.
Specimen 1C: embryonic day 14.5 (more )
Note: Triple labeled: green - Prdm16; white - Jag1 (assay MGI:7581992); red - Sox2 (assay MGI:7581990).
Structure Level Pattern Image Note
TS22: greater epithelial ridge Present Regionally restricted 1C Prdm16 , 1C Merge Expression was detected in Kolliker's organ. There was no co-localization with Jag1.
Specimen 2A Prdm16cGT/+: embryonic day 14.5; Prdm16Gt(OST67423)Lex/Prdm16+ (more )
Note: Double labeled: green - Prdm16; red - Sox2 (assay MGI:7581990).
Structure Level Pattern Image Note
TS22: greater epithelial ridge Present Regionally restricted 2A Prdm16cGT/+ Merge , 2A Prdm16cGT/+ Prdm16 , 2A Prdm16cGT/+ left Expression was detected in Kolliker's organ.
Specimen 2A Prdm16cGT/cGT: embryonic day 14.5; Prdm16Gt(OST67423)Lex/Prdm16Gt(OST67423)Lex (more )
Note: Double labeled: green - Prdm16; red - Sox2 (assay MGI:7581990).
Structure Level Pattern Image Note
TS22: greater epithelial ridge Absent 2A Prdm16cGT/cGT left , 2A Prdm16cGT/cGT Prdm16 , 2A Prdm16cGT/cGT Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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