Antibody:	anti-WAC
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2Aa

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cortical plate	cerebral cortex neuron	Present	Regionally restricted	2Ab	Perinuclear localization in cortical neurons. Moderate nuclear expression in limited number of cells.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Weak		2Ac	Relatively weak expression in intermediate zone.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	neural progenitor cell	Present		2Ad	Expression in progenitor cells in ventricular zone.

Structure	Level	Pattern	Image	Note
TS27: somatosensory cortex	Present	Regionally restricted	2Ba

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cortical plate	cerebral cortex neuron	Present	Regionally restricted	2Bb	Expression in nuclei of excitatory neurons throughout cortical plate. Not all neurons express.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cortical plate	cerebral cortex neuron	Present	Regionally restricted	2Bc	Expression in nuclei of excitatory neurons throughout cortical plate. Not all neurons express.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex intermediate zone	Weak		2Bd	Only weak expression in intermediate zone.

Structure	Level	Pattern	Image	Note
TS28: somatosensory cortex	Present	Regionally restricted	2Ca

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer II	cerebral cortex neuron	Weak		2Cb	Relatively weak expression in layer II/III neurons.
TS28: cortical layer III	cerebral cortex neuron	Weak		2Cb	Relatively weak expression in layer II/III neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer IV	cerebral cortex neuron	Present	Regionally restricted	2Cc	Expression in nuclei of neurons in cortical layers IV-VI.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer V	cerebral cortex neuron	Present	Regionally restricted	2Cd	Expression in nuclei of neurons in cortical layers IV-VI.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer VI	cerebral cortex neuron	Present	Regionally restricted	2Ce	Expression in nuclei of neurons in cortical layers IV-VI.

Structure	Level	Pattern	Image	Note
TS28: somatosensory cortex	Present	Regionally restricted	2Da

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer II	cerebral cortex neuron	Present	Regionally restricted	2Db	Expression enriched in nuclei of cortical neurons.
TS28: cortical layer III	cerebral cortex neuron	Present	Regionally restricted	2Db	Expression enriched in nuclei of cortical neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer IV	cerebral cortex neuron	Present	Regionally restricted	2Dc	Expression enriched in nuclei of cortical neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer V	cerebral cortex neuron	Present	Regionally restricted	2Dd	Expression enriched in nuclei of cortical neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cortical layer VI	cerebral cortex neuron	Present	Regionally restricted	2De	Expression enriched in nuclei of cortical neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: hippocampal formation	hippocampal neuron	Present	Regionally restricted	3Aa	Expression mainly enriched in nuclei at E17.

Structure	Level	Pattern	Image	Note
TS25: hippocampus	Present	Regionally restricted	3Ab

Structure	Level	Pattern	Image	Note
TS25: dentate gyrus	Present	Regionally restricted	3Ac

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present	Regionally restricted	3Ba	Expression accumulated densely in CA1, CA2 and neighboring lateral areas of CA3.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA1	hippocampal pyramidal neuron	Present	Regionally restricted	3Bb	Expression enriched in nucleus of pyramidal neurons in Cornu ammonis (CA)1-3.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	3Bc	Relatively sparce distribution of expression in dentate gyrus, especially in inner area, comared to outer area.

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Present	Regionally restricted	3Ca	Strong expression in medial side of CA1 and dentate gyrus. Expressing sparcely detected in lateral region of CA1, CA2 and CA3.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus CA1	hippocampal neuron	Strong	Regionally restricted	3Cb	Strong expression in medial side of CA1. Expression condensed in nuclei of neurons.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dentate gyrus	hippocampal neuron	Strong	Regionally restricted	3Cc	Strong expression in medial side of dentate gyrus. Expression condensed in nuclei of neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Diffuse	3Da	Expression somewhat diffusely distributed.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	hippocampal neuron	Present	Regionally restricted	3Db	Expression not accumulated in nucleus of neurons in molecular layer and granulal layer.
TS28: cerebellum molecular layer	hippocampal neuron	Present	Regionally restricted	3Db	Expression not accumulated in nucleus of neurons in molecular layer and granulal layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	3Ea

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	cerebellar basket cell	Present	Regionally restricted	3Eb	Expression in cells in molecular layer, assumed to be interneurons such as stellate cells and basket cells.
TS28: cerebellum molecular layer	cerebellar stellate cell	Present	Regionally restricted	3Eb	Expression in cells in molecular layer, assumed to be interneurons such as stellate cells and basket cells.
TS28: cerebellum granule cell layer	cerebellar granule cell	Present	Regionally restricted	3Eb	Expression in nuclei of Purkinje cells and granular cells.
TS28: cerebellum granule cell layer	Purkinje cell	Present	Regionally restricted	3Eb	Expression in nuclei of Purkinje cells and granular cells.
TS28: cerebellum white matter		Weak		3Eb	Little expression detected in cerebellar white matter.