Gene Expression Data
Assay Details
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Assay
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Reference:
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J:309045
Garcia-Guillen IM, et al., Netrin-1/DCC Signaling Differentially Regulates the Migration of Pax7, Nkx6.1, Irx2, Otp, and Otx2 Cell Populations in the Developing Interpeduncular Nucleus. Front Cell Dev Biol. 2020;8:588851
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7581074
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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Otx2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A:
embryonic day 18.5
(more  )
Note: Medial region. Double labeled: blue - Otx2; brown - Pax7 (assay MGI:7581075).
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Medial region. Double labeled: blue - Otx2; brown - Pax7 (assay MGI:7581075).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: interpeduncular nucleus |
Present |
Regionally restricted |
3A
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Expressed in the most medial part of the interpeduncular caudal region.
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Specimen
3B:
embryonic day 18.5
(more )
Note: Medio-lateral region. Double labeled: blue - Otx2; brown - Pax7 (assay MGI:7581075).
Specimen
3B:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Medio-lateral region. Double labeled: blue - Otx2; brown - Pax7 (assay MGI:7581075).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: interpeduncular nucleus |
Present |
Regionally restricted |
3B
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Expressed in the most medial part of the interpeduncular caudal region.
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Specimen
3C:
embryonic day 18.5
(more )
Note: Medial region.
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Medial region.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: interpeduncular nucleus |
Present |
Regionally restricted |
3C
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Expressed in the most medial part of the interpeduncular caudal region.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Dcctm1Wbg/Dcctm1Wbg
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Medial region.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: interpeduncular nucleus |
Present |
Regionally restricted |
3D
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A large part of the expression in the interpeduncular caudal region was absent, although some cells had reached their correct location in the caudal part.
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| TS26: hindbrain |
Present |
Patchy |
3D
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Expressed in patches of cells that appeared to have halted their migration to the interpeduncular nucleus.
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