Antibody:	Anti-Hsp27 antibody (ab1426)
Detection system:	Secondary antibody coupled to fluorescein

Structure	Cell Type	Level	Pattern	Image	Note
TS20: lens primordium	primary lens fiber	Weak		8A E12.5	Weakly expressed in the primary differentiating fiber cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lens fiber	lens fiber cell	Present		8A E14.5	Increased expression beginning with secondary fiber cell differentiation.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: lens fiber	lens fiber cell	Present	Regionally restricted	8A P0	Increased expression in the fiber cells in the center (possibly late fetal lens fiber cells).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens fiber	lens fiber cell	Present	Uniform	8A P15	Expressed fairly uniformly throughout the lens, both in the differentiating fiber cells at the lens periphery and in the inner, more mature fiber cells after they lose their nuclei.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens fiber	lens fiber cell	Present		8B control	Higher expression in the inner cortical fiber cells where it was associated with fiber cell membranes and remained associated with the fiber cell membranes even after they lost their nuclei.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: lens fiber	lens fiber cell	Present		8B -/-	Reduced expression in the mature lens fiber cells after they lost their nuclei.