Antibody:	Anti-WHSC1/NSD2 antibody [29D1] (ab75359)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS20: palatal shelf epithelium	Present	Regionally restricted	4A upper , 4B upper	Relatively weak expression in the nasal side.
TS20: palatal shelf mesenchyme	Present	Regionally restricted	4A upper , 4B upper	Relatively weak expression in the nasal side.
TS20: mandible primordium	Present		4A upper
TS20: nasal cavity epithelium	Present		4A upper
TS20: tongue	Present		4A upper
TS20: tooth	Present		4A upper

Structure	Level	Pattern	Image	Note
TS20: palatal shelf epithelium	Present	Regionally restricted	4A middle , 4B middle	Relatively weak expression in the nasal side.
TS20: palatal shelf mesenchyme	Present	Regionally restricted	4A middle , 4B middle	Relatively weak expression in the nasal side.
TS20: mandible primordium	Present		4A middle
TS20: tongue	Present		4A middle
TS20: tooth	Present		4A middle

Structure	Level	Pattern	Image	Note
TS20: palatal shelf epithelium	Present	Regionally restricted	4A lower , 4B lower	Relatively weak expression in the nasal side.
TS20: palatal shelf mesenchyme	Present	Regionally restricted	4A lower , 4B lower	Relatively weak expression in the nasal side.
TS20: mandible primordium	Present		4A lower
TS20: maxilla	Present		4A lower
TS20: tongue	Present		4A lower

Structure	Level	Pattern	Image	Note
TS21: palatal shelf epithelium	Strong	Regionally restricted	4C upper , 4D upper	Expression was reduced in the anterior palatal shelves and only strongly expressed in the region close to the nasal side.
TS21: palatal shelf mesenchyme	Strong	Regionally restricted	4C upper , 4D upper	Expression was reduced in the anterior palatal shelves and only strongly expressed in the region close to the nasal side.
TS21: nasal cavity epithelium	Present		4C upper
TS21: tongue	Present		4C upper

Structure	Level	Pattern	Image	Note
TS21: palatal shelf epithelium	Present		4C middle , 4D middle	Increased expression in the whole middle palatal shelves.
TS21: palatal shelf mesenchyme	Present		4C middle , 4D middle	Increased expression in the whole middle palatal shelves.
TS21: maxilla	Present		4C middle
TS21: tongue	Present		4C middle
TS21: tooth	Present		4C middle

Structure	Level	Pattern	Image	Note
TS21: palatal shelf epithelium	Present		4D lower , 4C lower	Increased expression in the whole posterior palatal shelves.
TS21: palatal shelf mesenchyme	Present		4D lower , 4C lower	Increased expression in the whole posterior palatal shelves.
TS21: mandible	Present		4C lower
TS21: tongue	Present		4C lower

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Strong	Regionally restricted	4F upper , 4E upper	Expressed in the epithelium close to the oral and nasal sides.
TS22: palatal shelf mesenchyme	Present	Widespread	4E upper , 4F upper	Relatively weak expression was seen in whole mesenchyme.
TS22: maxilla	Present		4E upper
TS22: nasal cavity epithelium	Present		4E upper
TS22: tongue	Present		4E upper

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Strong	Regionally restricted	4E middle , 4F middle	Expressed in the epithelium close to the oral and nasal sides.
TS22: palatal shelf mesenchyme	Present	Widespread	4F middle , 4E middle	Relatively weak expression was seen in whole mesenchyme.
TS22: maxilla	Present		4E middle
TS22: nasal cavity epithelium	Present		4E middle
TS22: tongue	Present		4E middle

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Strong		4E lower , 4F lower
TS22: palatal shelf mesenchyme	Present	Regionally restricted	4E lower , 4F lower	Mainly occurred in the tip of the palatal process.
TS22: nasal cavity epithelium	Present		4E lower
TS22: tongue	Present		4E lower
TS22: tooth	Present		4E lower

Structure	Level	Pattern	Image	Note
TS23: palatal shelf	Present	Regionally restricted	4G upper , 4H upper	Mainly expressed at points of fusion.
TS23: mandible	Present		4G upper
TS23: nasal cavity epithelium	Present		4G upper
TS23: tongue	Present		4G upper

Structure	Level	Pattern	Image	Note
TS23: palatal shelf	Present	Regionally restricted	4G middle , 4H middle	Mainly expressed at points of fusion.
TS23: tongue	Present		4G middle
TS23: tooth	Present		4G middle

Structure	Level	Pattern	Image	Note
TS23: palatal shelf	Present	Regionally restricted	4H lower , 4G lower	Mainly expressed at points of fusion.
TS23: maxilla	Present		4G lower
TS23: tongue	Present		4G lower

Structure	Level	Pattern	Image	Note
TS22: brain	Present		S2
TS22: dental papilla	Present		S2
TS22: enamel epithelium	Present		S2
TS22: mandible	Present		S2
TS22: maxilla	Present		S2
TS22: nasal cavity epithelium	Present		S2
TS22: tongue	Present		S2
TS22: hyaloid cavity	Present	Regionally restricted	S2	Expressed in the vitreous body.