Gene Expression Data
Assay Details
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Assay
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Reference:
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J:88676
Hammer F, et al., Transcriptional regulation of P450scc gene expression in the embryonic rodent nervous system. Endocrinology. 2004 Feb;145(2):901-12
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7579191
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Gene symbol:
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Sp4
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Gene name:
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trans-acting transcription factor 4
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Antibody:
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anti-Sp4
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
7 Sp4:
embryonic day 9.5
(more  )
Note: Double labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184).
Specimen
7 Sp4:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural ectoderm |
Present |
Regionally restricted |
7_Merged (lower)
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7_Sp4
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Expression co-localized with Cyp11a1 in ventral neurectoderm.
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| TS15: Rathke's pouch |
Present |
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7_Sp4
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7_Merged (lower)
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Expression co-localized with Cyp11a1 in Rathke's pouch.
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| TS15: branchial arch |
Present |
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7_Merged (lower)
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7_Sp4
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Expression co-localized with Cyp11a1 in branchial arches.
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Specimen
Sp4 E10.5 not shown:
embryonic day 10.5
(more )
Specimen
Sp4 E10.5 not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: migrating neural crest |
Present |
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Expression co-localized with Sp4 in cells that appear to be in position of cells migrating from neural crest.
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Specimen
8 Sp4 Cortex:
embryonic day 18.5
(more )
Note: Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
Specimen
8 Sp4 Cortex:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Regionally restricted |
8_Cortex Merged
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8_Cortex Sp4
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Co-localization of expression with Cyp11a1 and Sp1 in molecular layer of neocortex.
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| TS26: cortical plate |
Present |
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8_Cortex Merged
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8_Cortex Sp4
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Co-localization of expression with Cyp11a1 and Sp1 in cortical plate.
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Specimen
8 Sp4 Hippocampus:
embryonic day 18.5
(more )
Note: Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
Specimen
8 Sp4 Hippocampus:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hippocampus |
Present |
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8_Hippocampus Merged
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8_Hippocampus Sp4
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Co-localization of expression with Cyp11a1 and Sp1 in hippocampal neuroeipithelium.
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Specimen
8 Sp4 Cerebellum:
embryonic day 18.5
(more )
Note: Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
Specimen
8 Sp4 Cerebellum:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebellum Purkinje cell layer |
Present |
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8_Cerebellum Sp4
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8_Cerebellum Merged
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Co-localization of expression with Cyp11a1 and Sp1 in Purkinje cell layer.
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Specimen
8 Sp4 Retina:
embryonic day 18.5
(more )
Note: Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
Specimen
8 Sp4 Retina:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: retina ganglion cell layer |
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Present |
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8_Retina Sp4
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8_Retina Merged
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Co-localization of expression with Cyp11a1 and Sp1 in ganglion cell layer and inner nuclear layer of retina.
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| TS26: retina inner nuclear layer |
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Present |
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8_Retina Sp4
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8_Retina Merged
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Co-localization of expression with Cyp11a1 and Sp1 in ganglion cell layer and inner nuclear layer of retina.
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| TS26: retina |
amacrine cell |
Present |
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8_Retina Merged
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8_Retina Sp4
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Co-localization of expression with Cyp11a1 and Sp1 in amacrine cells of retina.
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Specimen
8 Sp4 Pituitary:
embryonic day 18.5
(more )
Note: Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
Specimen
8 Sp4 Pituitary:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pituitary gland |
Present |
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8_Pituitary Sp4
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8_Pituitary Merged
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Co-localization of expression with Cyp11a1 and Sp1 in pituitary primordium.
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Specimen
9 Sp4 Trigeminal Ganglion (upper):
embryonic day 18.5
(more )
Note: Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
Specimen
9 Sp4 Trigeminal Ganglion (upper):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: trigeminal ganglion |
Present |
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9_Trigeminal Ganglion Sp4 (upper)
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9_Trigeminal Ganglion Merged (upper)
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Co-localization of expression with Cyp11a1 and Sp1 in trigeminal ganglia.
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Specimen
9 Sp4 Trigeminal Ganglion (lowerr):
embryonic day 18.5
(more )
Note: Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
Specimen
9 Sp4 Trigeminal Ganglion (lowerr):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: trigeminal ganglion |
neuron |
Present |
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9_Trigeminal Ganglion Sp4 (lower)
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9_Trigeminal Ganglion Merged (lower)
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Co-localization of expression with Cyp11a1 and Sp1 in neurons.
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Specimen
9 Sp4 Dorsal Root Ganglion:
embryonic day 18.5
(more )
Note: Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
Specimen
9 Sp4 Dorsal Root Ganglion:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: dorsal root ganglion |
Present |
Regionally restricted |
9_Dorsal Root Ganglion Sp4
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9_Dorsal Root Ganglion Merged
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Co-localization of expression with Cyp11a1 and Sp1 in dorsal root ganglia.
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Specimen
9 Sp4 Whiskers:
embryonic day 18.5
(more )
Note: Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
Specimen
9 Sp4 Whiskers:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Sp4; green - Cyp11a1 (assay MGI:7579184); blue - Sp1 (assay MGI:7579189).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: vibrissa |
Present |
Regionally restricted |
9_Whiskers Sp4
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9_Whiskers Merged
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Co-localization of expression with Cyp11a1 in cortex.
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