About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:88676 Hammer F, et al., Transcriptional regulation of P450scc gene expression in the embryonic rodent nervous system. Endocrinology. 2004 Feb;145(2):901-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7579184
Gene symbol: Cyp11a1
Gene name: cytochrome P450, family 11, subfamily a, polypeptide 1
Antibody: anti-P450scc
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 3A P450scc Olfactory Bulb: embryonic day 18.5 (more )
Note: Double labeled: green - Cyp11a1; red - Xrcc5 (assay MGI:7579185).
Structure Level Pattern Image Note
TS26: main olfactory bulb Present Regionally restricted 3A Olfactory Bulb P450scc , 3A Olfactory Bulb Merged Expression co-localized with Xrcc5 in region of differentiating field of olfactory bulb.

Specimen 3A P450scc Cerebellum: embryonic day 18.5 (more )
Note: Double labeled: green - Cyp11a1; red - Xrcc5 (assay MGI:7579185).
Structure Cell Type Level Pattern Image Note
TS26: cerebellum Purkinje cell layer neuron Present 3A Cerebellum Merged , 3A Cerebellum P450scc Expression co-localized with Xrcc5 in Purkinje cell layer. Expression appears to be neuronal.

Specimen 3A P450scc Hindbrain: embryonic day 18.5 (more )
Note: Double labeled: green - Cyp11a1; red - Xrcc5 (assay MGI:7579185).
Structure Cell Type Level Pattern Image Note
TS26: inferior olivary nucleus neuron Present 3A Hindbrain P450scc , 3A Hindbrain Merged Expression co-localized with Xrcc5 in nucleus in position of inferior olive in basal plate of hindbrain (medulla). Expression appears to be neuronal.

Specimen 3A P450scc Nasal Epithelium: embryonic day 18.5 (more )
Note: Double labeled: green - Cyp11a1; red - Xrcc5 (assay MGI:7579185).
Structure Level Pattern Image Note
TS26: nasal epithelium Present Regionally restricted 3A Nasal Epithelium P450scc , 3A Nasal Epithelium Merged Expression co-localized with Xrcc5 in submucosa of nasal cavity.

Specimen 3B P450scc Cortex: embryonic day 18.5 (more )
Note: Double labeled: green - Cyp11a1; red - Xrcc6 (assay MGI:7579186).
Structure Cell Type Level Pattern Image Note
TS26: neocortex glial cell Present Regionally restricted 3B Cortx P450scc , 3B Cortex Merged Expression co-localized with Xrcc6 in molecular layer of cerebral cortex. Expression appears to be glial.
TS26: cerebral cortex intermediate zone Present 3B Cortx P450scc , 3B Cortex Merged Expression co-localized with Xrcc6 in cortical plate and intermediate layer of cerebral cortex.
TS26: cortical plate Present 3B Cortx P450scc , 3B Cortex Merged Expression co-localized with Xrcc6 in cortical plate and intermediate layer of cerebral cortex.

Specimen 3B P450scc Cerebellum: embryonic day 18.5 (more )
Note: Double labeled: green - Cyp11a1; red - Xrcc6 (assay MGI:7579186).
Structure Cell Type Level Pattern Image Note
TS26: cerebellum Purkinje cell layer neuron Present 3B Cerebellum P450scc , 3B Cerebellum Merged Expression co-localized with Xrcc6 in Purkinje cell layer. Expression appears to be neuronal.

Specimen 3B P450scc Hindbrain: embryonic day 18.5 (more )
Note: Double labeled: green - Cyp11a1; red - Xrcc6 (assay MGI:7579186).
Structure Cell Type Level Pattern Image Note
TS26: inferior olivary nucleus neuron Present 3B HIndbrain P450scc , 3B Hindbrain Merged Expression co-localized with Xrcc6 in a nucleus in the position of the inferior olive. Expression appears to be neuronal.

Specimen P450scc E18.5 not shown: embryonic day 18.5 (more )
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex Present Regionally restricted Expression co-localized with Xrcc5 in cerebral cortex.
TS26: spinal cord motor neuron Present Expression co-localized with Xrcc5 in motor neurons in spinal cord.
TS26: nasal epithelium Present Regionally restricted Expression co-localized with Xrcc6 in both epithelial cells and glandular mucosal cells of nasal epithelium.

Specimen 7 P450scc (upper): embryonic day 9.5 (more )
Note: Double labeled: green - Cyp11a1; red - Sp1 (assay MGI:7579188).
Structure Level Pattern Image Note
TS15: embryo Present Regionally restricted 7_P450scc (upper) , 7_Merged (upper) No co-localization of expression with Sp1 in any region.

Specimen 7 P450scc (middle): embryonic day 9.5 (more )
Note: Double labeled: green - Cyp11a1; red - Sp2 (assay MGI:7579190).
Structure Level Pattern Image Note
TS15: neural ectoderm Present Regionally restricted 7_Merged (middle) , 7_P450scc (middle) Expression co-localized with Sp2 in ventral neurectoderm.
TS15: neural tube Present Regionally restricted 7_P450scc (middle) , 7_Merged (middle) Expression co-localized with Sp2 in ventral neural tube.
TS15: branchial arch Present 7_Merged (middle) , 7_P450scc (middle) Expression co-localized with Sp2 in branchial arches.

Specimen 7 P450scc (lower): embryonic day 9.5 (more )
Note: Double labeled: green - Cyp11a1; red - Sp4 (assay MGI:7579191).
Structure Level Pattern Image Note
TS15: neural ectoderm Present Regionally restricted 7_Merged (lower) , 7_P450scc (lower) Expression co-localized with Sp4 in ventral neurectoderm.
TS15: Rathke's pouch Present 7_P450scc (lower) , 7_Merged (lower) Expression co-localized with Sp4 in Rathke's pouch.
TS15: branchial arch Present 7_P450scc (lower) , 7_Merged (lower) Expression co-localized with Sp4 in branchial arches.

Specimen P450scc E10.5 not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: migrating neural crest Present Expression co-localized with Sp4 in cells that appear to be in position of cells migrating from neural crest.

Specimen 8 P450scc Cortex: embryonic day 18.5 (more )
Note: Triple labeled: green - Cyp11a1; blue - Sp1 (assay MGI:7579189); red - Sp4 (assay MGI:7579191).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex glial cell Present Regionally restricted 8_Cortex P450scc , 8_Cortex Merged Co-localization of expression with Sp1 and Sp4 in molecular layer of neocortex. Expression appears to be glial.
TS26: cortical plate Present 8_Cortex P450scc , 8_Cortex Merged Co-localization of expression with Sp1 and Sp4 in cortical plate.

Specimen 8 P450scc Hippocampus: embryonic day 18.5 (more )
Note: Triple labeled: green - Cyp11a1; blue - Sp1 (assay MGI:7579189); red - Sp4 (assay MGI:7579191).
Structure Level Pattern Image Note
TS26: hippocampus Present 8_Hippocampus Merged , 8_Hippocampus P450scc Co-localization of expression with Sp1 and Sp4 in hippocampal neuroepithelium.

Specimen 8 P450scc Cerebellum: embryonic day 18.5 (more )
Note: Triple labeled: green - Cyp11a1; blue - Sp1 (assay MGI:7579189); red - Sp4 (assay MGI:7579191).
Structure Level Pattern Image Note
TS26: cerebellum Purkinje cell layer Present 8_Cerebellum Merged , 8_Cerebellum P450scc Co-localization of expression with Sp1 and Sp4 in Purkinje cell layer of cerebellum.

Specimen 8 P450scc Retina: embryonic day 18.5 (more )
Note: Triple labeled: green - Cyp11a1; blue - Sp1 (assay MGI:7579189); red - Sp4 (assay MGI:7579191).
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer Present 8_Retina P450scc , 8_Retina Merged Co-localization of expression with Sp1 and Sp4 in ganglion cell layer and inner nuclear layer.
TS26: retina inner nuclear layer Present 8_Retina P450scc , 8_Retina Merged Co-localization of expression with Sp1 and Sp4 in ganglion cell layer and inner nuclear layer.
TS26: retina amacrine cell Present 8_Retina P450scc , 8_Retina Merged Co-localization of expression with Sp1 and Sp4 in amacrine cells of retina.

Specimen 8 P450scc Pituitary: embryonic day 18.5 (more )
Note: Triple labeled: green - Cyp11a1; blue - Sp1 (assay MGI:7579189); red - Sp4 (assay MGI:7579191).
Structure Level Pattern Image Note
TS26: pituitary gland Present 8_Pituitary P450scc , 8_Pituitary Merged Co-localization of expression with Sp1 and Sp4 in pituitary primordium.

Specimen 9 P450scc Trigeminal Ganglion (upper): embryonic day 18.5 (more )
Note: Triple labeled: green - Cyp11a1; blue - Sp1 (assay MGI:7579189); red - Sp4 (assay MGI:7579191).
Structure Level Pattern Image Note
TS26: trigeminal ganglion Present 9_Trigeminal Ganglion Merged (upper) , 9_Trigeminal Ganglion P450scc (upper) Co-localization of expression with Sp1 and Sp4 in trigeminal ganglia.

Specimen 9 P450scc Trigeminal Ganglion (lower): embryonic day 18.5 (more )
Note: Triple labeled: green - Cyp11a1; blue - Sp1 (assay MGI:7579189); red - Sp4 (assay MGI:7579191).
Structure Cell Type Level Pattern Image Note
TS26: trigeminal ganglion neuron Present 9_Trigeminal Ganglion Merged (lower) , 9_Trigeminal Ganglion P450scc (lower) Co-localization of expression with Sp1 and Sp4 in neurons.

Specimen 9 P450scc Dorsal Root Ganglion: embryonic day 18.5 (more )
Note: Triple labeled: green - Cyp11a1; blue - Sp1 (assay MGI:7579189); red - Sp4 (assay MGI:7579191).
Structure Level Pattern Image Note
TS26: dorsal root ganglion Present 9_Dorsal Root Ganglion Merged , 9_Dorsal Root Ganglion P450scc Co-localization of expression with Sp1 and Sp4 in dorsal root ganglia.

Specimen 9 P450scc Whiskers: embryonic day 18.5 (more )
Note: Triple labeled: green - Cyp11a1; blue - Sp1 (assay MGI:7579189); red - Sp4 (assay MGI:7579191).
Structure Level Pattern Image Note
TS26: vibrissa outer root sheath Present 9_Whiskers Merged , 9_Whiskers P450scc Co-localization of expression with Sp1 in external root sheath.
TS26: vibrissa Present Regionally restricted 9_Whiskers Merged , 9_Whiskers P450scc Co-localization of expression with Sp4 in cortex.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
04/16/2024
MGI 6.23
The Jackson Laboratory