Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:242842
Kharfallah F, et al., Scribble1 plays an important role in the pathogenesis of neural tube defects through its mediating effect of Par-3 and Vangl1/2 localization. Hum Mol Genet. 2017 Jun 15;26(12):2307-2320
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7579155
|
|
Gene symbol:
|
Pard3
|
|
Gene name:
|
par-3 family cell polarity regulator
|
|
Antibody:
|
anti-Par3 (07-330)
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 555
|
|
|
Results
|
Specimen
1J:
embryonic day 9.0-9.5
(more  )
Specimen
1J:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.0-9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: neural tube |
Present |
Regionally restricted |
1J (middle)
,
1J (upper)
,
1J (lower)
|
Expression used as apical marker.
|
Specimen
1K:
(close )
|
Genetic Background:
|
involves: C57BL/6J * NMRI
|
|
Mutant Allele(s):
|
ScribCrc/ScribCrc
|
|
Age:
|
embryonic day 9.0-9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: neural tube |
Present |
Regionally restricted |
1K (lower)
,
1K (middle)
,
1K (upper)
|
Localization at apical site lost and pattern changed from cell borders to mostly cytosolic.
|
Specimen
S1J:
embryonic day 11.5
(more )
Specimen
S1J:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube |
Present |
Regionally restricted |
S1J (lower)
,
S1J (middle)
,
S1J (upper)
|
Expression used as apical marker.
|
Specimen
S1K:
(close )
|
Genetic Background:
|
involves: C57BL/6J * NMRI
|
|
Mutant Allele(s):
|
ScribCrc/ScribCrc
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube |
Present |
Regionally restricted |
S1K (upper)
,
S1K (lower)
,
S1K (middle)
|
Cellular distribution of expression severely disrupted compared to wild type.
|
|
Analysis Tools