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Gene Expression Data
Assay Details
Assay
Reference: J:316994 Lefebvre J, et al., Alternatively spliced isoforms of WT1 control podocyte-specific gene expression. Kidney Int. 2015 Aug;88(2):321-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7576941
Gene symbol: Nphs1
Gene name: nephrosis 1, nephrin
Results
Specimen S1A NPHS1/WT1 Control: embryonic day 17.5 (more )
Note: Double labeled: green - Nphs1; red - Wt1 (assay MGI:7576943).
Structure Level Pattern Image Note
TS25: metanephros Present S1A Control
Specimen S1A NPHS1/WT1 (-KTS-/-): embryonic day 17.5; Wt1tm1Asc/Wt1tm1Asc (more )
Note: Double labeled: green - Nphs1; red - Wt1 (assay MGI:7576943).
Structure Level Pattern Image Note
TS25: metanephros Absent S1A (-KTS-/-)
Specimen S1A NPHS1/WT1 (+KTS-/-): embryonic day 17.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Double labeled: green - Nphs1; red - Wt1 (assay MGI:7576943).
Structure Level Pattern Image Note
TS25: metanephros Present Regionally restricted S1A (+KTS-/-) Abnormal localization pattern.
Specimen S1B NPHS1/WT1 Control: embryonic day 17.5 (more )
Note: Double labeled: green - Nphs1; red - Wt1 (assay MGI:7576943).
Structure Level Pattern Image Note
TS25: metanephros Present S1B Control NPHS1/WT1 inset , S1B Control NPHS1/WT1
Specimen S1B NPHS1/WT1 (-KTS-/-): embryonic day 17.5; Wt1tm1Asc/Wt1tm1Asc (more )
Note: Double labeled: green - Nphs1; red - Wt1 (assay MGI:7576943).
Structure Level Pattern Image Note
TS25: metanephros Absent S1B (-KTS-/-) NPHS1/WT1 , S1B (-KTS-/-) NPHS1/WT1 inset
Specimen S1B NPHS1/WT1 (+KTS-/-): embryonic day 17.5; Wt1tm2Asc/Wt1tm2Asc (more )
Note: Double labeled: green - Nphs1; red - Wt1 (assay MGI:7576943).
Structure Level Pattern Image Note
TS25: metanephros Present Regionally restricted S1B (+KTS-/-) NPHS1/WT1 , S1B (+KTS-/-) NPHS1/WT1 inset Abnormal localization pattern.
Specimen 4d Control: postnatal adult (more )
Structure Level Pattern Image Note
TS28: metanephros Present 4d Control
Specimen 4d Magi2 ko: postnatal adult; Magi2tm1Yhat/Magi2tm1Yhat (more )
Structure Level Pattern Image Note
TS28: metanephros Trace 4d Magi2 ko Expression markedly reduced compared to control.
Specimen 4e Control: postnatal adult (more )
Note: Triple labeled: green - Nphs1; red - Magi2 (assay MGI:7576942); blue - Wt1 (assay MGI:7576943).
Structure Level Pattern Image Note
TS28: metanephros Present 4e Control NPHS1 , 4e Control inset , 4e Control (merged)
Specimen 4e Magi2 ko: postnatal adult; Magi2tm1Yhat/Magi2tm1Yhat (more )
Note: Triple labeled: green - Nphs1; red - Magi2 (assay MGI:7576942); blue - Wt1 (assay MGI:7576943).
Structure Level Pattern Image Note
TS28: metanephros Weak Regionally restricted 4e Magi2 ko (merged) , 4e Magi2 ko NPHS1 , 4e Magi2 ko inset Punctuated and cytoplasmic expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory