Wnt1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316482 Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
Assay type:	RNA in situ
MGI Accession ID:	MGI:7576064
Gene symbol:	Wnt1
Gene name:	wingless-type MMTV integration site family, member 1

Probe:	Wnt1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

7H +/+ left ©

Specimen 7H +/+ lateral to medial 1: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present	Regionally restricted	7H +/+ left	Expressed in the isthmic organizer.

Specimen 7H +/+ lateral to medial 2: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present	Regionally restricted	7H +/+ middle	Expressed in the isthmic organizer.

7H +/+ right ©

Specimen 7H +/+ lateral to medial 3: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present	Regionally restricted	7H +/+ right	Expressed in the isthmic organizer.
TS20: midbrain	Present		7H +/+ right

Specimen 7H LacZ/LacZ lateral to medial 1: embryonic day 12.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present	Regionally restricted	7H LacZ/LacZ left	Expressed in the isthmic organizer, similar as in the wild-type.

Specimen 7H LacZ/LacZ lateral to medial 2: embryonic day 12.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present	Regionally restricted	7H LacZ/LacZ middle	Expressed in the isthmic organizer, similar as in the wild-type.

Specimen 7H LacZ/LacZ lateral to medial 3: embryonic day 12.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Present	Regionally restricted	7H LacZ/LacZ right	Expressed in the isthmic organizer, similar as in the wild-type.
TS20: midbrain	Present		7H LacZ/LacZ right	Similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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