En1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316482 Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
Assay type:	RNA in situ
MGI Accession ID:	MGI:7576056
Gene symbol:	En1
Gene name:	engrailed 1

Probe:	En1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

7B +/+ left ©

Specimen 7B +/+ lateral to medial 1: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		7B +/+ left

Specimen 7B +/+ lateral to medial 2: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		7B +/+ middle

7B +/+ right ©

Specimen 7B +/+ lateral to medial 3: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		7B +/+ right

Specimen 7B LacZ/LacZ lateral to medial 1: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		7B LacZ/LacZ left	Similar as in the wild-type.

Specimen 7B LacZ/LacZ lateral to medial 2: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		7B LacZ/LacZ middle	Similar as in the wild-type.

Specimen 7B LacZ/LacZ lateral to medial 3: embryonic day 14.5; Nkx2-9^tm1Jlck/Nkx2-9^tm1Jlck (more

)

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present		7B LacZ/LacZ right	Similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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