Gene Expression Data
Assay Details
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Assay
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Reference:
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J:316482
Kouwenhoven WM, et al., Nkx2.9 Contributes to Mid-Hindbrain Patterning by Regulation of mdDA Neuronal Cell-Fate and Repression of a Hindbrain-Specific Cell-Fate. Int J Mol Sci. 2021 Nov 23;22(23)
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7576026
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Gene symbol:
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Nkx2-9
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Gene name:
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NK2 homeobox 9
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Reporter:
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lacZ
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Antibody:
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anti-B-galactosidase
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Results
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Specimen
3B lateral to medial 1:
embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more  )
Specimen
3B lateral to medial 1:
(close  )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B lateral to medial 2:
embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Specimen
3B lateral to medial 2:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B lateral to medial 3:
embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Specimen
3B lateral to medial 3:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B lateral to medial 4:
embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Specimen
3B lateral to medial 4:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B lateral to medial 5:
embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Specimen
3B lateral to medial 5:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B lateral to medial 6:
embryonic day 12.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Specimen
3B lateral to medial 6:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3D lateral to medial 1:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Specimen
3D lateral to medial 1:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
3D-1
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Expressed in mesodiencephalic dopaminergic neuron area. Expressed in the ventral midbrain abutting the mesencephalic flexure.
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Specimen
3D lateral to medial 2:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Specimen
3D lateral to medial 2:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
3D-2
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Expressed in mesodiencephalic dopaminergic neuron area. Expressed in the ventral midbrain abutting the mesencephalic flexure.
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Specimen
3D lateral to medial 3:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Specimen
3D lateral to medial 3:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
3D-3
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Expressed in mesodiencephalic dopaminergic neuron area. Expressed in the ventral midbrain abutting the mesencephalic flexure.
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Specimen
3D lateral to medial 4:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Specimen
3D lateral to medial 4:
(close )
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Genetic Background:
|
involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
|
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
3D-4
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Expressed in mesodiencephalic dopaminergic neuron area. Expressed in the ventral midbrain abutting the mesencephalic flexure.
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| TS22: hindbrain |
Present |
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3D-4
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Specimen
3D lateral to medial 5:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Specimen
3D lateral to medial 5:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
Regionally restricted |
3D-5
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Expressed in mesodiencephalic dopaminergic neuron area. Expressed in the ventral midbrain abutting the mesencephalic flexure.
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| TS22: hindbrain |
Present |
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3D-5
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Specimen
3F lateral to medial 1:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Specimen
3F lateral to medial 1:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
3F left
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Coexpressed with Th in mesodiencephalic dopaminergic neurons in the rostral-lateral midbrain. Th+beta-gal- neurons were interspersed with double-positive ones.
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Specimen
3F lateral to medial 2:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Specimen
3F lateral to medial 2:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
3F middle left
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Coexpressed with Th in mesodiencephalic dopaminergic neurons in the rostral-lateral midbrain. Th+beta-gal- neurons were interspersed with double-positive ones.
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Specimen
3F lateral to medial 3:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Specimen
3F lateral to medial 3:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
3F middle right
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Coexpressed with Th in mesodiencephalic dopaminergic neurons in the caudomedial midbrain. Th+beta-gal- neurons were interspersed with double-positive ones.
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Specimen
3F lateral to medial 4:
embryonic day 14.5; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Specimen
3F lateral to medial 4:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
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Cryosection
|
|
Note:
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Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
midbrain dopaminergic neuron |
Present |
Regionally restricted |
3F right
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Coexpressed with Th in mesodiencephalic dopaminergic neurons in the caudomedial midbrain. Th+beta-gal- neurons were interspersed with double-positive ones.
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Specimen
3H SNc:
postnatal adult; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Specimen
3H SNc:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
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Specimen
3H VTA:
postnatal adult; Nkx2-9tm1Jlck/Nkx2-9 +
(more )
Note: Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
Specimen
3H VTA:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
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Cryosection
|
|
Note:
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Double labeled: red - Nkx2-9 reporter; green - Th (assay MGI:7576029).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: ventral tegmental area |
midbrain dopaminergic neuron |
Present |
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3H VTA
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Both Th+beta-gal+ and Th+beta-gal- neurons were present.
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Specimen
4D lateral to medial 1:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
|
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Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
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Age:
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embryonic day 14.5
|
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Sex:
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Not Specified
|
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Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4D lateral to medial 2:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
|
|
Mutant Allele(s):
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Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
|
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Age:
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embryonic day 14.5
|
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Sex:
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Not Specified
|
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Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4D lateral to medial 3:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4D lateral to medial 4:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4D lateral to medial 5:
(close )
|
Genetic Background:
|
involves: 129/Sv * C57BL/6 * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Nkx2-9tm1Jlck/Nkx2-9tm1Jlck
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
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Analysis Tools