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Gene Expression Data
Assay Details
Assay
Reference: J:316190 Lacko LA, et al., Novel expression of EGFL7 in placental trophoblast and endothelial cells and its implication in preeclampsia. Mech Dev. 2014 Aug;133:163-76
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7574736
Gene symbol: Egfl7
Gene name: EGF-like domain 7
Antibody: Anti-EGFL7 (R-12) sc-34416
Assay notes: Three antibodies were described: MGI:7574622, MGI:3580175, and MGI:7574624. The one used in this assay was not specified.
Results
Specimen 1G: postnatal adult (more )
Note: Double labeled: red - Egfl7; green - Pecam1 (assay MGI:7574737).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell of placenta Present 1I , 1G Expression co-localized with the endothelial cell marker, Pecam1 in the maternal decidua.
TS28: decidua endothelial cell of placenta Present 1I , 1G Expression co-localized with the endothelial cell marker, Pecam1 in the maternal decidua.

Specimen 1K: embryonic day 12.5 (more )
Note: Double labeled: red - Egfl7; green - Pecam1 (assay MGI:7574737).
Structure Cell Type Level Pattern Image Note
TS20: extraembryonic vascular system endothelial cell of placenta Present Regionally restricted 1M , 1K Expression co-localized with the endothelial cell marker, Pecam1 in the fetal endothelial cells of the placenta.
TS20: labyrinthine zone endothelial cell of placenta Present Regionally restricted 1M , 1K Expression co-localized with the endothelial cell marker, Pecam1 in the fetal endothelial cells of the placenta.

Specimen 2C: embryonic day 10.5 (more )
Note: Double labeled: green - Egfl7; red - CK.
Structure Cell Type Level Pattern Image Note
TS17: junctional zone trophoblast cell Present 2F , 2C , 2E Expression was co-localized with the pan-trophoblast marker, Cytokeratin in the junctional zone.

Specimen 2H: embryonic day 3.5-4.0 (more )
Note: Blastocyst stage. Double labeled: red - Egfl7; green - Cdx2 (assay MGI:7574744).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2H , 2J
TS4: trophectoderm Present 2H , 2J Expression co-localized with the trophectoderm cell marker, Cdx2.

Specimen 4C: embryonic day 10.5 (more )
Note: Double labeled: red - Egfl7; green - Pecam1 (assay MGI:7574737).
Structure Level Pattern Image Note
TS17: extraembryonic vascular system Present 4C , 4E
TS17: labyrinthine zone Present 4C , 4E

Specimen 4G: embryonic day 10.5 (more )
Note: Double labeled: red - Egfl7; green - Pecam1 (assay MGI:7574737).
Structure Level Pattern Image Note
TS17: extraembryonic vascular system Weak 4I , 4G Expression was reduced in the fetal labyrinth zone of the BPH/5 placentas compared to C57BL/6.
TS17: labyrinthine zone Weak 4I , 4G Expression was reduced in the fetal labyrinth zone of the BPH/5 placentas compared to C57BL/6.

Specimen S2A maternal decidua: postnatal adult (more )
Note: Double labeled: red - Egfl7; green - Pecam1 (assay MGI:7574737).
Structure Cell Type Level Pattern Image Note
TS28: blood vessel endothelial cell of placenta Present S2A Merge top , S2A EGFL7 top Expression co-localized with the endothelial cell marker, Pecam1 in the maternal decidua.
TS28: decidua endothelial cell of placenta Present S2A Merge top , S2A EGFL7 top Expression co-localized with the endothelial cell marker, Pecam1 in the maternal decidua.

Specimen S2A Fetal labyrinth: embryonic day 10.5 (more )
Note: Double labeled: red - Egfl7; green - Pecam1 (assay MGI:7574737).
Structure Cell Type Level Pattern Image Note
TS17: extraembryonic vascular system endothelial cell of placenta Present S2A EGFL7 bottom , S2A Merge bottom Expression co-localized with the endothelial cell marker, Pecam1 in the fetal endothelial cells of the placenta.
TS17: labyrinthine zone endothelial cell of placenta Present S2A EGFL7 bottom , S2A Merge bottom Expression co-localized with the endothelial cell marker, Pecam1 in the fetal endothelial cells of the placenta.

Specimen S2B: embryonic day 18.5 (more )
Note: Double labeled: red - Egfl7; green - Pecam1 (assay MGI:7574737).
Structure Cell Type Level Pattern Image Note
TS26: extraembryonic vascular system endothelial cell of placenta Present S2B Merge , S2B EGFL7 Expression co-localized with the endothelial cell marker, Pecam1 in the fetal endothelial cells of the placenta.
TS26: labyrinthine zone endothelial cell of placenta Present S2B Merge , S2B EGFL7 Expression co-localized with the endothelial cell marker, Pecam1 in the fetal endothelial cells of the placenta.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory