Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:128359
Oh HJ, et al., PIAS1 interacts with and represses SOX9 transactivation activity. Mol Reprod Dev. 2007 Nov;74(11):1446-55
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Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:7574629
|
Gene symbol:
|
Sox9
|
Gene name:
|
SRY (sex determining region Y)-box 9
|
Antibody:
|
anti-Sox9
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 594
|
|
Results
|
Specimen
2B:
embryonic day 13.0
(more )
Note: Double labeled: red - Sox9; green - Pias1 (assay MGI:7574628).
Specimen
2B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox9; green - Pias1 (assay MGI:7574628).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
2F
,
2B
,
2E
,
2C
|
Expression co-localized with Pias1. Sox9 was primarily located in Sertoli cells within and around the testicular cord.
|
Specimen
3A:
embryonic day 15.0
(more )
Note: Double labeled: red - Sox9; green - Pias1 (assay MGI:7574628).
Specimen
3A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 15.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Sox9; green - Pias1 (assay MGI:7574628).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cartilage |
Present |
|
3B
,
3A
,
3G
,
3E
,
3D
|
Expression was detected in the cartilage primordium, and co-localized with Pias1 in dot-like structures within the positive nuclei.
|
|