Gene Expression Data
Assay Details
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Assay
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Reference:
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J:128359
Oh HJ, et al., PIAS1 interacts with and represses SOX9 transactivation activity. Mol Reprod Dev. 2007 Nov;74(11):1446-55
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7574628
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Gene symbol:
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Pias1
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Gene name:
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protein inhibitor of activated STAT 1
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Antibody:
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Anti-Pias1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2A:
embryonic day 13.0
(more  )
Note: Double labeled: green - Pias1; red - Sox9 (assay MGI:7574629).
Specimen
2A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pias1; red - Sox9 (assay MGI:7574629).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS21: Sertoli cell |
Sertoli cell |
Present |
|
2D
,
2A
,
2F
,
2C
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Expression co-localized with Sox9. Pias1 was positive within a few dot-like structures in the Sertoli cells.
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| TS21: germ cell of testis |
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Present |
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2F
,
2D
,
2C
,
2A
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| TS21: mesonephros of male |
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Present |
Ubiquitous |
2A
,
2D
,
2C
,
2F
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Expression was ubiquitous in the nuclei of cells with the gonad and mesonephros.
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| TS21: testis |
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Present |
Ubiquitous |
2A
,
2D
,
2C
,
2F
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Expression was ubiquitous in the nuclei of cells with the gonad and mesonephros.
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Specimen
3C:
embryonic day 15.0
(more )
Note: Double labeled: green - Pias1; red - Sox9 (assay MGI:7574629).
Specimen
3C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pias1; red - Sox9 (assay MGI:7574629).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cartilage |
Present |
Ubiquitous |
3G
,
3D
,
3C
,
3F
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Expression was ubiquitous in the cartilage primordium, and co-localized with Sox9 in dot-like structures within the positive nuclei.
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Analysis Tools