Antibody:

clone RC2

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cerebral cortex	forebrain radial glial cell	Present	Regionally restricted	4A	Immunofluorescence, marking radial glial cell processes, was observed along cell processes with ventricular-to-pial orientation, essentially parallel to each other.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cerebral cortex	forebrain radial glial cell	Present	Regionally restricted	4B	Immunolabeled radial glial fibres in the C3G<gt/gt> mutants were disorganised, lacked ventricular-to-pial orientation and were entangled. Areas of overlap between laminin and Nes were similar in number in C3G<gt/gt> mutants compared with control at E12.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: cerebral cortex	forebrain radial glial cell	Present	Regionally restricted	4C	Immunofluorescence, marking radial glial cell processes, was observed along cell processes with ventricular-to-pial orientation, essentially parallel to each other.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: cerebral cortex	forebrain radial glial cell	Present	Regionally restricted	4D	Immunolabeled radial glial fibres in the C3G<gt/gt> mutants were disorganised, lacked ventricular-to-pial orientation and were entangled.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: cerebral cortex	forebrain radial glial cell	Present	Regionally restricted	4E	Numerous small areas of overlap were seen with laminin basement membrane staining, representing anchoring of radial glial fibers.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: cerebral cortex	forebrain radial glial cell	Present	Regionally restricted	4F	Areas of overlap between Nes and laminin were significantly reduced in the mutants compared with wild type.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cerebral cortex	forebrain radial glial cell	Present	Regionally restricted	4G	Numerous small areas of overlap were seen with laminin basement membrane staining, representing anchoring of radial glial fibers.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cerebral cortex	forebrain radial glial cell	Present	Regionally restricted	4H	Areas of overlap between Nes and laminin were significantly reduced in the mutants compared with wild type.