Vim Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:131173 Liu Y, et al., Zeb1 links epithelial-mesenchymal transition and cellular senescence. Development. 2008 Feb;135(3):579-88
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7572955
Gene symbol:	Vim
Gene name:	vimentin

Antibody:	anti-vimentin
Detection system:	Secondary antibody coupled to Cy3

Results

1C ©

Specimen 1C: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: nasal mesenchyme	Present	1C
TS24: palate mesenchyme	Present	1C
TS24: tongue muscle	Present	1C

1E ©

Specimen 1E: embryonic day 16.5; Zeb1^tm2Yhi/Zeb1^tm2Yhi (more

)

Structure	Level	Pattern	Image	Note
TS24: nasal mesenchyme	Absent		1E
TS24: palate mesenchyme	Absent		1E

2B ©

Specimen 2B: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: perichondrium	Present		2B

2H ©

Specimen 2H: embryonic day 15.5; Zeb1^tm2Yhi/Zeb1^tm2Yhi (more

)

Structure	Level	Pattern	Image	Note
TS23: perichondrium	Absent		2H

3C ©

Specimen 3C: embryonic day 16.5 (more

)

Structure	Level	Image
TS24: eye muscle	Present	3C
TS24: lens	Present	3C
TS24: optic nerve	Present	3C
TS24: retina	Present	3C

3F ©

Specimen 3F: embryonic day 16.5; Zeb1^tm2Yhi/Zeb1^tm2Yhi (more

)
Note: Double labeled: red - Vim; green - Gfap (assay MGI:7572957).

Structure	Level	Image
TS24: eye muscle	Absent	3F
TS24: optic nerve	Absent	3F
TS24: retina	Absent	3F

4B ©

Specimen 4B: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Present	Regionally restricted	4B	Expressed in the ventricular zone of the lateral ventricle.

4D ©

Specimen 4D: embryonic day 15.5; Zeb1^tm2Yhi/Zeb1^tm2Yhi (more

)
Note: Double labeled: red - Vim; green - Gfap (assay MGI:7572957).

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Ambiguous		4D	Expression was lost in the ventricular zone of the lateral ventricle.

S1A ©

Specimen S1A: embryonic day 16.5; Zeb1^tm2Yhi/Zeb1^tm2Yhi (more

)

Structure	Level	Pattern	Image	Note
TS24: tongue muscle	Present		S1A	Similar as in the wild-type.

S1B ©

Specimen S1B: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: tongue muscle	Present		S1B

S1C ©

Specimen S1C: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: tongue muscle	Present		S1C

S3D ©

Specimen S3D: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: olfactory lobe	Present		S3D

S3E ©

S3F ©

Specimen S3E/F: embryonic day 15.5; Zeb1^tm2Yhi/Zeb1^tm2Yhi (more

)
Note: Samples should be from the mutant, although labeled as +/+.

Structure	Level	Pattern	Image	Note
TS23: olfactory lobe	Ambiguous		S3E , S3F	Loss of expression (either trace or absent) was seen.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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