Sncatm1.1(SNCA*)Ge
Targeted Allele Detail
|
|
| Symbol: |
Sncatm1.1(SNCA*)Ge |
| Name: |
synuclein, alpha; targeted mutation 1.1, Gregor Eichele |
| MGI ID: |
MGI:7572833 |
| Synonyms: |
halphaSynTP |
| Gene: |
Snca Location: Chr6:60708559-60806839 bp, - strand Genetic Position: Chr6, 29.15 cM
|
| Alliance: |
Sncatm1.1(SNCA*)Ge page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:314280
|
| Parent Cell Line: |
Not Specified (ES Cell)
|
| Strain of Origin: |
129
|
|
| Allele Type: |
|
Targeted (Humanized sequence, Inserted expressed sequence) |
| Mutation: |
|
Insertion
|
| |
|
Sncatm1.1(SNCA*)Ge expresses
1 gene
Knock-in expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
SNCA (6622) |
|
Expresses mutant human SNCA with three alanine to proline substitutions |
|
| |
|
Mutation details: A targeting vector consisting of loxP-flanked human alpha-synuclein, followed by human alpha-synuclein with three alanine to proline substitutions at residues 30, 56, and 76 (p.A30P, p.A56P, p.A76P), IRES, a placental alkaline phosphatase (pLAP) reporter gene, and an FRT-flanked neomycin gene was targeted to exon 3. The neomycin selection cassette was removed via Flp-mediated recombination. Cre-mediated recombination removed the floxed human alpha-synuclein resulting in the expression of the mutated human protein. RT-PCR confirmed the expression of the expected 566 bp human mutant transcript. RT-PCR also confirmed the absence of the wild-type human alpha-synuclein and expression of the mutant human alpha-synuclein transcript. The triple alanine to proline substitutions favors oligomer formation.
(J:314280)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Snca Mutation: |
42 strains or lines available
|
|
| Original: |
J:314280 Martinez Hernandez A, et al., Low-Expressing Synucleinopathy Mouse Models Based on Oligomer-Forming Mutations and C-Terminal Truncation of alpha-Synuclein. Front Neurosci. 2021;15:643391 |
| All: |
1 reference(s) |
|
Analysis Tools
