Antibody:	anti-Ki67 (TEC-3; M7249)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Two Mki67 antibodies were described, but the one used in this assay was not detailed. Anti-Ki67 (NCL-Ki67p) MGI:2154446 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial groove ectoderm	Present	Regionally restricted	2B	Along branchial clefts, groups of proliferating surface ectoderm cells are arranged in a roughly alternating pattern with senescent cells.
TS17: 2nd branchial arch ectoderm	Present	Regionally restricted	2B	Expressing cells dominate in all other parts of branchial arch surface ectoderm.
TS17: 2nd branchial arch mesenchyme	Present	Regionally restricted	2B	Proliferating cells predominate in mesenchymal core of branchial arches.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial groove ectoderm	Present	Regionally restricted	2D	Along branchial clefts, groups of proliferating surface ectoderm cells are arranged in a roughly alternating pattern with senescent cells.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial arch mesenchyme	Present	Regionally restricted	2F	Proliferating cells predominate in mesenchymal core of branchial arches.

Structure	Level	Pattern	Image	Note
TS17: petrosal placode	Present	Regionally restricted	6E Ki67	Authors report that central parts of placodal pit dominated by proliferating cells.

Structure	Level	Pattern	Image	Note
TS17: petrosal placode	Present	Regionally restricted	6F Ki67	Authors report that central parts of placodal pit dominated by proliferating cells.

Structure	Level	Pattern	Image	Note
TS17: petrosal placode	Present	Regionally restricted	6G Ki67	Authors report that central parts of placodal pit dominated by proliferating cells.

Structure	Level	Pattern	Image	Note
TS17: petrosal placode	Present	Regionally restricted	6H Ki67	Authors report that central parts of placodal pit dominated by proliferating cells.

Structure	Level	Pattern	Image	Note
TS14: 2nd branchial pouch endoderm	Present	Regionally restricted	16A Ki67	Rostral walls of pharyngeal pouches show massive proliferation.

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial pouch endoderm	Present	Clusters	16B Ki67	Expression predominantly in cell clusters.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial pouch endoderm	Weak	Clusters	16C Ki67	Clusters of expressing endoderm cells. Expression decreases over time.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial pouch endoderm	Weak	Clusters	16D Ki67	Clusters of expressing endoderm cells. Expression decreases over time.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial pouch endoderm	Weak	Clusters	16E Ki67	Clusters of expressing endoderm cells. Expression decreases over time.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial pouch endoderm	Weak	Clusters	16F Ki67	Clusters of expressing endoderm cells. Expression decreases over time.