Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Chrna6tm2(flpo)Tasic
Name:	cholinergic receptor, nicotinic, alpha polypeptide 6; targeted mutation 2, Bosiljka Tasic
MGI ID:	MGI:7571383
Synonyms:	Chrna6-IRES2-FlpO-neo
Gene:	Chrna6  Location: Chr8:27893240-27903972 bp, - strand  Genetic Position: Chr8, 16.02 cM, cytoband A3
Alliance:	Chrna6tm2(flpo)Tasic page

Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Chrna6tm2(flpo)Tasic expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Chrna6 (MGI:106213)   Mutation details: The targeting vector contains, from 5' to 3', a partial Chrna6 sequence within exon 6 up to and including the endogenous stop codon, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a FlpO recombinase gene (a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an AttP site. (J:101977)

Activity:	Tissue activity of this recombinase allele
Driver:	Chrna6 (mouse) Summary of all recombinase alleles driven by Chrna6. Alliance mouse Chrna6 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Chrna6 Mutation:	27 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	1 reference(s)