Nefm Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:203998 Luxey M, et al., Eph:ephrin-B1 forward signaling controls fasciculation of sensory and motor axons. Dev Biol. 2013 Nov 15;383(2):264-74
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7571351
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Antibody:	anti-neurofilament clone 2H3
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

1H' ©

Specimen 1H': embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb nerve	Present		1H'

1I ©

Specimen 1I: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb nerve	Present		1I

S1A ©

S1A''' ©

Specimen S1A: embryonic day 12.5 (more

)
Note: Triple labeled: blue - Nefm; green - Efnb1 (assay MGI:7571347); red - Epha4 (assay MGI:7571350).

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		S1A''' , S1A

S1D ©

Specimen S1D: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		S1D

S1E ©

Specimen S1E: embryonic day 12.5 (more

)
Note: Double labeled: red - Nefm; green - Efnb1 (assay MGI:7571347).

Structure	Level	Pattern	Image	Note
TS20: forelimb nerve	Present		S1E

4A ©

4A' ©

Specimen 4A,A': embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb nerve	Present	Regionally restricted	4A , 4A'	Expression in peripheral projections in dermis.

4B ©

4B' ©

Specimen 4B,B': embryonic day 12.5; Efnb1^tm1.1Sor/Efnb1^tm1.1Sor (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb nerve	Present	Regionally restricted	4B , 4B'	Perpheral projections exhibited exuberant branching.

S2A ©

Specimen S2A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: dermis	Present	Regionally restricted	S2A	Expression in peripheral projections in dermis.

S2B ©

Specimen S2B: embryonic day 12.5; Efnb1^tm1.1Sor/Efnb1^tm1.1Sor (more

)

Structure	Level	Pattern	Image	Note
TS20: dermis	Present	Regionally restricted	S2B	Peripheral projections exhibited exuberant branching.

S2C ©

Specimen S2C: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present		S2C

S2D ©

Specimen S2D: embryonic day 10.5; Efnb1^tm1.1Sor/Efnb1^tm1.1Sor (more

)

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present	Regionally restricted	S2D	Motor and sensory defasciculated branches in proximal and distal regions of nerves.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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