Trp53 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:283328 Klimovich B, et al., Inactivation of Mdm2 restores apoptosis proficiency of cooperativity mutant p53 in vivo. Cell Cycle. 2020 Jan;19(1):109-123
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7569304
Gene symbol:	Trp53
Gene name:	transformation related protein 53

Antibody:	anti-p53 (CM5) (NCL-p53-505)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Specimen 3a Mdm2+/-: embryonic day 10.5; Mdm2^tm1.1Mep/Mdm2⁺, Trp53^tm1.1Thst/Trp53^tm1.1Thst (more

)

Structure	Level	Image
TS17: embryo	Absent	3a Mdm2+/-;Trp53RR/RR
TS17: brain	Absent	3a Mdm2+/-;Trp53RR/RR , 3a +/- (1)
TS17: heart	Absent	3a Mdm2+/-;Trp53RR/RR , 3a +/- (2)
TS17: future spinal cord	Absent	3a +/- (3) , 3a Mdm2+/-;Trp53RR/RR
TS17: somite	Absent	3a Mdm2+/-;Trp53RR/RR , 3a +/- (4)
TS17: limb bud	Absent	3a Mdm2+/-;Trp53RR/RR , 3a +/- (5)
TS17: embryo mesenchyme	Absent	3a Mdm2+/-;Trp53RR/RR , 3a +/- (6)

Specimen 3a Mdm2-/-: embryonic day 10.5; Mdm2^tm1.1Mep/Mdm2^tm1.1Mep, Trp53^tm1.1Thst/Trp53^tm1.1Thst (more

)

Structure	Level	Pattern	Image	Note
TS17: embryo	Strong	Regionally restricted	3a Mdm2-/-;Trp53RR/RR	Strong accumulation of expression in all tissues except heart.
TS17: brain	Strong		3a Mdm2-/-;Trp53RR/RR , 3a -/- (1)	Strong expression particularly in neuroepithelium, limb buds and somites.
TS17: heart	Weak		3a -/- (2) , 3a Mdm2-/-;Trp53RR/RR	Markedly lower expression in cardiac tissues.
TS17: future spinal cord	Strong		3a Mdm2-/-;Trp53RR/RR , 3a -/- (3)	Strong expression particularly in neuroepithelium, limb buds and somites.
TS17: somite	Strong		3a -/- (4) , 3a Mdm2-/-;Trp53RR/RR	Strong expression particularly in neuroepithelium, limb buds and somites.
TS17: limb bud	Strong		3a -/- (5) , 3a Mdm2-/-;Trp53RR/RR	Strong expression particularly in neuroepithelium, limb buds and somites.
TS17: embryo mesenchyme	Present		3a -/- (6) , 3a Mdm2-/-;Trp53RR/RR

Specimen 4e Mdm2+/-: embryonic day 10.5; Cdkn1a^tm1Tyj/Cdkn1a^tm1Tyj, Mdm2^tm1.1Mep/Mdm2⁺, Trp53^tm1.1Thst/Trp53^tm1.1Thst (more

)

Structure	Level	Image
TS17: embryo	Absent	4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/-
TS17: brain	Absent	4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/- , 4e +/- (1)
TS17: heart	Absent	4e +/- (2) , 4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/-
TS17: future spinal cord	Absent	4e +/- (3) , 4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/-
TS17: somite	Absent	4e +/- (4) , 4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/-
TS17: limb bud	Absent	4e +/- (5) , 4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/-
TS17: embryo mesenchyme	Absent	4e Mdm2+/-;Trp53RR/RR;Cdkn1a-/- , 4e +/- (6)

Specimen 4e Mdm2-/-: embryonic day 10.5; Cdkn1a^tm1Tyj/Cdkn1a^tm1Tyj, Mdm2^tm1.1Mep/Mdm2^tm1.1Mep, Trp53^tm1.1Thst/Trp53^tm1.1Thst (more

)

Structure	Level	Pattern	Image	Note
TS17: embryo	Strong	Regionally restricted	4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/-	Authors report strong accumulation of expression.
TS17: brain	Strong		4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/- , 4e -/- (1)
TS17: heart	Present		4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/- , 4e -/- (2)
TS17: future spinal cord	Strong		4e -/- (3) , 4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/-
TS17: somite	Strong		4e -/- (4) , 4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/-
TS17: limb bud	Strong		4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/- , 4e -/- (5)
TS17: embryo mesenchyme	Strong		4e -/- (6) , 4e Mdm2-/-;Trp53RR/RR;Cdkn1a-/-

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24