Gene Expression Data
Assay Details
Assay
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Reference:
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J:272567
Aires R, et al., Tail Bud Progenitor Activity Relies on a Network Comprising Gdf11, Lin28, and Hox13 Genes. Dev Cell. 2019 Feb 11;48(3):383-395.e8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7568802
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Gene symbol:
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Lin28b
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Gene name:
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lin-28 homolog B
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Probe:
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Lin28b cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
|
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Results
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Specimen
5Bb:
embryonic day 10.5
(more )
Specimen
5Bb:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
Age:
|
embryonic day 10.5
|
Sex:
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Not Specified
|
Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: tail neural tube |
Present |
Regionally restricted |
5Bb inset
,
5Bb
|
Expressed in the caudal part.
|
TS17: tail mesenchyme |
Present |
Regionally restricted |
5Bb
,
5Bb inset
|
Expressed in the ventral mesoderm adjacent the neural tube.
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Specimen
5Bf:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gdf11tm1Sjl/Gdf11tm1Sjl
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: tail mesenchyme |
Present |
|
5Bf
,
5Bf inset
|
Stronger expression compared with the wild-type.
|
TS17: tail neural tube |
Present |
|
5Bf
,
5Bf inset
|
Stronger expression compared with the wild-type.
|
Specimen
5Bj:
embryonic day 11.5
(more )
Specimen
5Bj:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: tail mesenchyme |
Weak |
Regionally restricted |
5Bj
,
5Bj inset
|
Strongly down-regulated expression in the entire tail region. An absence of any detectable transcripts in the tip region was seen.
|
TS19: tail neural tube |
Weak |
Regionally restricted |
5Bj
,
5Bj inset
|
Strongly down-regulated expression in the entire tail region. An absence of any detectable transcripts in the tip region was seen.
|
Specimen
5Bn:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
Mutant Allele(s):
|
Gdf11tm1Sjl/Gdf11tm1Sjl
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
|