Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:171473
Nie X, et al., Six1 regulates Grem1 expression in the metanephric mesenchyme to initiate branching morphogenesis. Dev Biol. 2011 Apr 1;352(1):141-51
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7567725
|
|
Gene symbol:
|
Tbx18
|
|
Gene name:
|
T-box18
|
|
Probe:
|
Tbx18 probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
3A:
embryonic day 11.25
(more  )
Specimen
3A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.25
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: urinary system mesenchyme |
Strong |
Regionally restricted |
3A
|
Expressed in the ureteral mesenchyme. Expression domain was surrounding the ureteric bud trunk region and ventral to the metanephric mesenchyme, where ureteric bud normally invaded.
|
| TS19: metanephric mesenchyme |
Absent |
|
3A
|
|
Specimen
3B:
(close )
|
Genetic Background:
|
involves: 129/SvEv * 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 11.25
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: urinary system mesenchyme |
Present |
|
3B
|
The elongating ureter/ureteric bud tip was embedded within the Tbx18-expressing mesenchyme.
|
Specimen
3C:
embryonic day 11.5
(more )
Specimen
3C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: urinary system mesenchyme |
Strong |
Regionally restricted |
3C
|
Expressed in the ureteral mesenchyme. Expression domain was surrounding the ureteric bud trunk region and ventral to the metanephric mesenchyme, where ureteric bud normally invaded.
|
| TS19: metanephric mesenchyme |
Absent |
|
3C
|
|
Specimen
3D:
(close )
|
Genetic Background:
|
involves: 129/SvEv * 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: urinary system mesenchyme |
Present |
|
3D
|
The elongating ureter/ureteric bud tip was embedded within the Tbx18-expressing mesenchyme.
|
Specimen
3E:
embryonic day 12.5
(more )
Specimen
3E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3F:
(close )
|
Genetic Background:
|
involves: 129/SvEv * 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Analysis Tools