Antibody:	Anti-Hsp104
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Present	Regionally restricted	1D	Expression was detected in mesectoderm.
TS15: neural tube	Present		1D

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present		1E	Expression was detected in numerous cells located in the dorsal part of the first two branchial arches.
TS15: 2nd branchial arch	Present		1E	Expression was detected in numerous cells located in the dorsal part of the first two branchial arches.
TS15: 1st branchial arch mandibular component	Present		1E
TS15: 1st branchial arch maxillary component	Present		1E
TS15: embryo mesenchyme	Present	Regionally restricted	1E	Expression was detected in mesectoderm.
TS15: oral region	Present	Regionally restricted	1E	Expression was detected in dental lamina.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	1F	Expression was detected in mesenchymal cells ventral to the otic vesicle.
TS15: embryo	Present	Regionally restricted	1F	Expression was detected in the epiblast.
TS15: future hindbrain	Present	Regionally restricted	1F	Expression was detected in neural epithelium of the rhombencephalon.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	2E	Expression was detected in more distal mesectodermal cells, as compared with E9.5, within the mandibular bud.
TS17: 1st branchial arch maxillary component	Present		2E
TS17: oral region	Present	Regionally restricted	2E	Expression was detected in the dental lamina.

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Present	Regionally restricted	2F	Compared with E9.5, the expression at E10.5 has progressed in a more distal (ventral) direction. Expression was detected in dorsal mesectodermal cells and in epiblast.
TS17: central nervous system	Present		2F	Expression was detected in the neural epithelium.