Antibody:

anti-TLE4

Structure	Cell Type	Level	Pattern	Image	Note
TS17: neural tube		Present	Regionally restricted	7a	Expression was highest in the nuclei of cells in the ventral horn regions of the rostral neural tube. Weaker immunoreactivity was observed in apical cells of the dorsal neural tube.
TS17: neural tube	central nervous system neuron	Present		7a	Expression was observed in both progenitor cells and differentiated neurons of the neural tube.
TS17: sclerotome		Present		7a

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		7c
TS20: forebrain	Present		7c
TS20: medulla oblongata	Present		7c
TS20: midbrain	Present		7c
TS20: pons	Present		7c

Structure	Cell Type	Level	Pattern	Image	Note
TS22: diencephalon		Present		7d
TS22: hypothalamus		Present	Regionally restricted	7d	Expression was highest in the area likely corresponding to the ventromedial hypothalamic nucleus.
TS22: cerebral cortex	cerebral cortex neuron	Present	Regionally restricted	7d	Expression was detected in the more internal layers of differentiated cortical neurons.
TS22: cortical plate		Strong	Regionally restricted	7d , 7e	Expression was strong in the inner layers of of the cortical plate.
TS22: choroid plexus		Strong		7d , 7e
TS22: cerebral cortex ventricular layer		Weak		7e , 7d

Structure	Cell Type	Level	Pattern	Image	Note
TS22: cerebral cortex	cerebral cortex neuron	Present	Regionally restricted	8a	Highest immunoreactivity was observed in cortical neurons of more internal layers.
TS22: choroid plexus		Present		8a
TS22: cerebral cortex intermediate zone		Weak		8a
TS22: cerebral cortex ventricular layer		Weak		8a

Structure	Cell Type	Level	Pattern	Image	Note
TS24: cortical plate	cerebral cortex neuron	Present	Regionally restricted	8b	Expression marked the older neurons of the internal layers of the cortical plate.
TS24: cerebral cortex ventricular layer		Weak		8b
TS24: cerebral cortex subventricular zone		Absent		8b

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Strong	Regionally restricted	9c	Expression was strongest in cells of the ventral half of the spinal cord.
TS22: dorsal root ganglion	Strong		9c
TS22: spinal cord ventricular layer	Weak		9c

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Strong		9d	Robust immunoreactivity was observed in the majority of cells.