Antibody:	mAb 5750
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS21: spinal cord lumen	Strong		1B	Expression was locally defined signal at the central canal.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Strong	Widespread	1D	Expression increased and changed to a more diffuse, widespread staining.
TS23: spinal cord lumen	Present		1D

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present	Widespread	1F	Expression was diffuse throughout the spinal cord.
TS26: spinal cord lumen	Present		1F

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present		4E

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present		4F	Expression was not affected in the mutant.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present		5C left , 5C right
TS23: spinal cord lumen	Present		5C right , 5C left	Possibly overlapping areas of expression with Ptprz1 were observed around the central canal and the future white matter.
TS23: spinal cord white matter	Present		5C right , 5C left	Possibly overlapping areas of expression with Ptprz1 were observed around the central canal and the future white matter.