Antibody:	mAb 487
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS21: spinal cord lumen	Present		1A	Expression was locally defined signal at the central canal.
TS21: spinal cord mantle layer	Present	Regionally restricted	1A	Expression was detected in the ventral mantle zone.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Strong	Widespread	1C	Expression increased and changed to a more diffuse, widespread staining.
TS23: spinal cord lumen	Weak		1C

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present	Widespread	1E	Expression was diffuse throughout the spinal cord.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present		4C

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present		4D	Expression was not affected in the mutant.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present		5D left , 5D right
TS23: spinal cord lumen	Present		5D right , 5D left	Possibly overlapping areas of expression with Ptprz1 were observed around the central canal and the future white matter.
TS23: spinal cord white matter	Present		5D right , 5D left	Possibly overlapping areas of expression with Ptprz1 were observed around the central canal and the future white matter.