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Gene Expression Data
Assay Details
Assay
Reference: J:84406 Hikita ST, et al., Integrin alpha4beta1 (VLA-4) expression and activity in retinal and peripheral neurons. Mol Cell Neurosci. 2003 Jul;23(3):427-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7564322
Gene symbol: Itga4
Gene name: integrin alpha 4
Antibody: anti-alpha 4 integrin
Detection system: Secondary antibody
Results
Specimen 1A/C: embryonic day 15.0 (more )
Note: The same pattern of staining was observed using the anti-alpha4 monoclonal antibody 9C10. Double labeled: green - Itga4; red - Tubb3 (assay MGI:7564323).
Structure Cell Type Level Pattern Image Note
TS23: retina nuclear layer retinal ganglion cell Present 1A , 1C
TS23: lens Present 1A , 1C
TS23: optic nerve Present 1A , 1C
TS23: retina nerve fiber layer Present 1A , 1C
TS23: hyaloid cavity hyalocyte Present 1C , 1A
TS23: retina nuclear layer retinoblast Strong 1C , 1A Strong expression in the peripheral tips of the retina, which contain the least differentiated cells.
TS23: vitreous humour Present Clusters 1A , 1C
Specimen 1D/F: embryonic day 15.0 (more )
Note: The same pattern of staining was observed using the anti-alpha4 monoclonal antibody 9C10. Double labeled: green - Itga4; red - Tubb3 (assay MGI:7564323).
Structure Cell Type Level Pattern Image Note
TS23: vitreous humour hyalocyte Present Clusters 1F , 1D
TS23: retina nuclear layer retinal ganglion cell Present 1F , 1D
TS23: retina nuclear layer retinoblast Present 1F , 1D
Specimen 2A/C: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Itga4; red - Itgb1 (assay MGI:7564324).
Structure Cell Type Level Pattern Image Note
TS23: retina nuclear layer retinal ganglion cell Present 2C , 2A
TS23: retina nuclear layer retinoblast Present 2C , 2A
TS23: hyaloid vascular plexus Present 2A , 2C
TS23: hyaloid cavity hyalocyte Present 2C , 2A
TS23: lens Present 2A , 2C
TS23: optic nerve Present 2A , 2C
TS23: retina nerve fiber layer Present 2A , 2C
Specimen 2D/F: embryonic day 15.0-16.0 (more )
Note: Double labeled: green - Itga4; red - Ricinus communis agglutinin I lectin.
Structure Level Pattern Image Note
TS23: hyaloid vascular plexus Present 2F , 2D Costaining with Ricinus communis agglutinin I lectin.
Specimen 3A/D: embryonic day 15.0 (more )
Note: Triple labeled: green - Itga4; red - Itgb1 (assay MGI:7564325); blue - Spp1 (assay MGI:7564326).
Structure Level Pattern Image Note
TS23: hyaloid cavity Present 3D , 3A
TS23: lens Present 3D , 3A
TS23: optic nerve Present 3D , 3A
TS23: retina nerve fiber layer Present 3A , 3D
TS23: retina nuclear layer Present 3D , 3A
Specimen 3E/H: embryonic day 15.0 (more )
Note: Triple labeled: green - Itga4; red - Itgb1 (assay MGI:7564325); blue - Spp1 (assay MGI:7564326).
Structure Cell Type Level Pattern Image Note
TS23: retina nerve fiber layer Present 3H , 3E
TS23: vitreous humour Present 3H , 3E
TS23: retina nuclear layer retinal ganglion cell Present 3H , 3E
TS23: retina nuclear layer retinoblast Present 3H , 3E
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory