Antibody:	anti-Suv39h2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 568

Structure	Level	Pattern	Image	Note
TS28: spermatid	Present		8A	Expressed in clustered centromeres in round spermatids but was no longer detectable in elongating spermatids.

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Present		8C	Expressed in late, but not early, spermatocytes in a subnuclear region, reminiscent of the XY body.
TS28: spermatid	Present		8C	Expressed in clustered centromeres in round spermatids but was no longer detectable in elongating spermatids.

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present		9A PL	Expressed as a granular stain in all preleptotene nuclei.

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Weak		9A eSP	Expressed weakly but distinctly apparent at blocks of heterochromatin.

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Present		9A mSP	Expressed at the sex chromosomes present in the XY body.

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Present		9A lSP	Expressed at the sex chromosomes present in the XY body.

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Weak		9A dSP	Expressed weakly but distinctly apparent at blocks of heterochromatin.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Present		9A rSTs	Expressed at the condensing heterochromatic foci and chromocenters of haploid spermatids.

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Present		9B Suv39h2 , 9B merge	Colocalizes with Slx/Xmr at the sex chromosomes present in the XY body.

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Present			Expressed in a dispersed distribution in some premeiotic nuclei.
TS28: spermatozoon	Absent