Gene Expression Data
Assay Details
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Assay
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Reference:
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J:128304
Petrou P, et al., Basement membrane localization of Frem3 is independent of the Fras1/Frem1/Frem2 protein complex within the sublamina densa. Matrix Biol. 2007 Oct;26(8):652-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7564017
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Gene symbol:
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Frem1
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Gene name:
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Fras1 related extracellular matrix protein 1
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Antibody:
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anti-Frem1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Assay notes:
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A Tyramide signal amplification step was used.
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Results
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Specimen
1B:
embryonic day 14.5
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Present |
Regionally restricted |
1B
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Expression is in the epithelial basement membrane of the skin.
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| TS22: skin |
Present |
Regionally restricted |
1B
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Expression is in the epithelial basement membrane of the skin.
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Specimen
1G:
embryonic day 14.5
(more )
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Present |
Regionally restricted |
1G
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Expression is in the epithelial basement membrane of the developing airways and visceral pleura in the lung.
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| TS22: lung |
Present |
Regionally restricted |
1G
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Expression is in the epithelial basement membrane of the developing airways and visceral pleura in the lung.
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| TS22: visceral pleura |
Present |
Regionally restricted |
1G
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Expression is in the epithelial basement membrane of the developing airways and visceral pleura in the lung.
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Specimen
1L:
embryonic day 14.5
(more )
Specimen
1L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Present |
Regionally restricted |
1L
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Expression is in the epithelial basement membrane of the kidney.
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| TS22: metanephros |
Present |
Regionally restricted |
1L
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Expression is in the epithelial basement membrane of the kidney.
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Specimen
2A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fras1tm1Chpk/Fras1tm1Chpk
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Absent |
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2A
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Expression in the epithelial basement membrane of the skin in this mutant is absent.
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| TS22: skin |
Absent |
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2A
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Expression in the epithelial basement membrane of the skin in this mutant is absent.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fras1tm1Chpk/Fras1tm1Chpk
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Absent |
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2B
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Expression in the epithelial basement membrane of the lung in this mutant is absent.
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| TS22: lung |
Absent |
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2B
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Expression in the epithelial basement membrane of the lung in this mutant is absent.
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fras1tm1Chpk/Fras1tm1Chpk
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: basement membrane of epithelium |
Trace |
Regionally restricted |
2C
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Expression in the epithelial basement membrane of renal tubules.
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| TS22: renal tubule |
Trace |
Regionally restricted |
2C
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Expression in the epithelial basement membrane of renal tubules.
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Analysis Tools