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Gene Expression Data
Assay Details
Assay
Reference: J:342123 Sterling NA, et al., P53 independent pathogenic mechanisms contribute to BubR1 microcephaly. Front Cell Dev Biol. 2023;11:1282182
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7548372
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-gammaH2AX (Ser139) (05-636)
Detection system: Secondary antibody
Results
Specimen 3G gH2AX WT: embryonic day 14.5 (more )
Note: Double labeled: green - H2ax; red - phospho-histone H3.
Structure Level Pattern Image Note
TS22: cerebral cortex Ambiguous 3G WT , 3G WT inset Authors report a lack of significant DNA damage in wild type and Trp53 cKO groups.
Specimen 3G gH2AX Trp53 cKO: embryonic day 14.5; Emx1tm1(cre)Krj/Emx1+, Trp53tm1Brn/Trp53tm1Brn (more )
Note: Conditional mutant. Double labeled: green - H2ax; red - phospho-histone H3.
Structure Level Pattern Image Note
TS22: cerebral cortex Ambiguous 3G Trp53 cKO inset , 3G Trp53 cKO Authors report a lack of significant DNA damage in wild type and Trp53 cKO groups.
Specimen 3G gH2AX BubR1 cKO: embryonic day 14.5; Bub1btm1Jvd/Bub1btm1Jvd, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - H2ax; red - phospho-histone H3.
Structure Level Pattern Image Note
TS22: cerebral cortex Strong 3G BubR1 cKO , 3G BubR1 cKO inset Number of expressing cells signficantly increased compared to wild type.
Specimen 3G gH2AX dcKO: embryonic day 14.5; Bub1btm1Jvd/Bub1btm1Jvd, Emx1tm1(cre)Krj/Emx1+, Trp53tm1Brn/Trp53tm1Brn (more )
Note: Conditional mutant. Double labeled: green - H2ax; red - phospho-histone H3.
Structure Level Pattern Image Note
TS22: cerebral cortex Strong 3G dcKO inset , 3G dcKO Number of expressing cells not altered compared to BubR1 cKO.
Specimen 3J gH2AX WT: embryonic day 14.5 (more )
Note: Double labeled: green - H2ax; red - Casp3 (assay MGI:7548368).
Structure Level Pattern Image Note
TS22: cerebral cortex Ambiguous 3J WT Authors report a lack of significant DNA damage in wild type and Trp53 cKO groups.
Specimen 3J gH2AX Trp53 cKO: embryonic day 14.5; Emx1tm1(cre)Krj/Emx1+, Trp53tm1Brn/Trp53tm1Brn (more )
Note: Conditional mutant. Double labeled: green - H2ax; red - Casp3 (assay MGI:7548368).
Structure Level Pattern Image Note
TS22: cerebral cortex Ambiguous 3J Trp53 cKO Authors report a lack of significant DNA damage in wild type and Trp53 cKO groups.
Specimen 3J gH2AX BubR1 cKO: embryonic day 14.5; Bub1btm1Jvd/Bub1btm1Jvd, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: green - H2ax; red - Casp3 (assay MGI:7548368).
Structure Level Pattern Image Note
TS22: cerebral cortex Strong Regionally restricted 3J BubR1 cKO Significant number of expressing cells are undergoing apoptosis.
Specimen 3J gH2AX dcKO: embryonic day 14.5; Bub1btm1Jvd/Bub1btm1Jvd, Emx1tm1(cre)Krj/Emx1+, Trp53tm1Brn/Trp53tm1Brn (more )
Note: Conditional mutant. Double labeled: green - H2ax; red - Casp3 (assay MGI:7548368).
Structure Level Pattern Image Note
TS22: cerebral cortex Strong 3J dcKO Number of expressing cells not altered compared to BubR1 cKO.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory