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Gene Expression Data
Assay Details
Assay
Reference: J:311540 Oliver BL, et al., Spatial and temporal expression of PORCN is highly dynamic in the developing mouse cochlea. Gene Expr Patterns. 2021 Sep 20;42:119214
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7547384
Gene symbol: Porcn
Gene name: porcupine O-acyltransferase
Antibody: anti-Porcn (PA5-43423)
Detection system: Secondary antibody
Results
Specimen 4A: embryonic day 14.5; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+, Porcntm1.1Lcm/Porcn+ (more )
Note: Conditional mutant. Control.
Structure Level Pattern Image Note
TS22: cochlear duct Present Non-Uniform 4A Expression is enriched in the medial domain.
Specimen 4B: embryonic day 14.5; Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+, Porcntm1.1Lcm/Y (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: cochlea Absent 4B
Specimen 5A: embryonic day 12.5 (more )
Note: Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS20: cochlear duct Present Non-Uniform 5A Expression is seen in the basal turn of the cochlea. It is broadly expressed across the radial axis and enriched centrally in the floor of the duct.
Specimen 5B: embryonic day 13.5 (more )
Note: Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS21: cochlear duct Present Graded 5B , 5B' Expression is seen in a basal to apical gradient.
Specimen 5C: embryonic day 14.5 (more )
Note: base t1. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS22: cochlear duct Present Non-Uniform 5C Expression is highest in the base across the radial axis but then becomes restricted to the medial domain toward the apex.
Specimen 5D: embryonic day 14.5 (more )
Note: mid t2. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS22: cochlear duct Present Non-Uniform 5D Expression is highest in the base across the radial axis but then becomes restricted to the medial domain toward the apex.
Specimen 5E: embryonic day 14.5 (more )
Note: apex t3. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS22: cochlear duct Present Non-Uniform 5E Expression is highest in the base across the radial axis but then becomes restricted to the medial domain toward the apex.
Specimen 6A: embryonic day 16.5 (more )
Note: base t1. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS24: cochlear duct Weak Regionally restricted 6A Low levels of expression are seen in the inner hair cells and neighboring supporting cells; there is no expression in the far medial domain.
Specimen 6B: embryonic day 16.5 (more )
Note: mid t2. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS24: cochlear duct Weak Regionally restricted 6B Low levels of expression are seen in the inner hair cells and neighboring supporting cells; there is no expression in the far medial domain.
Specimen 6B': embryonic day 16.5 (more )
Note: mid t2. Triple labeled: green - Porcn; red - Sox2 (assay MGI:7547373); blue - DAPI.
Structure Level Pattern Image Note
TS24: cochlear duct Present Non-Uniform 6B' Expression is enriched in the domain containing the inner hair cells.
Specimen 6C: embryonic day 16.5 (more )
Note: mid t3. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS24: cochlear duct Present 6C Expression is seen in the medial compartment.
Specimen 6D: embryonic day 16.5 (more )
Note: apex t4. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS24: cochlear duct Present 6D There is an enrichment of expression in the medial domain; it does not extend across the radial axis.
Specimen 6E: postnatal day 1 (more )
Note: base t1. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS27: cochlear duct Present Regionally restricted 6E Expression is seen on the basolateral side of the inner chair cells and in the Dieters' and Hensens' cells.
Specimen 6F: postnatal day 1 (more )
Note: mid t2. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS27: cochlear duct Present Regionally restricted 6F Expression is seen on the basolateral side of the inner chair cells and in the Dieters' and Hensens' cells.
Specimen 6F': postnatal day 1 (more )
Note: mid t2. Triple labeled: green - Porcn; red - Sox2 (assay MGI:7547373); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: cochlear duct cochlear inner hair cell Present 6F'
TS27: cochlear duct Deiter's cell Present 6F'
TS27: cochlear duct Hensen cell Present 6F'
Specimen 6G: postnatal day 1 (more )
Note: mid t3. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS27: cochlear duct Trace 6G
Specimen 6H: postnatal day 1 (more )
Note: apex t4. Double labeled: green - Porcn; magenta - Sox2 (assay MGI:7547373).
Structure Level Pattern Image Note
TS27: cochlear duct Trace 6H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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