Gene Expression Data
Assay Details
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Assay
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Reference:
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J:110095
Ligon KL, et al., Development of NG2 neural progenitor cells requires Olig gene function. Proc Natl Acad Sci U S A. 2006 May 16;103(20):7853-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7547381
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Gene symbol:
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Cspg4
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Gene name:
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chondroitin sulfate proteoglycan 4
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Antibody:
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anti-NG2 (AB5320)
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
3A +/-:
embryonic day 14.5; Olig2tm1Rth/Olig2 +
(more  )
Specimen
3A +/-:
(close  )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3A -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
pericyte |
Present |
Regionally restricted |
3A -/-
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Only staining in vessel pericytes was unaffected. No scattered positive neural progenitor cells with synantocyte or bipolar morphology were seen.
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Specimen
3B +/- 10x:
embryonic day 18.5; Olig2tm1Rth/Olig2 +
(more )
Specimen
3B +/- 10x:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B -/- 10x:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: brain |
pericyte |
Present |
Regionally restricted |
3B 10X -/-
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Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.
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Specimen
3B +/- CX:
embryonic day 18.5; Olig2tm1Rth/Olig2 +
(more )
Specimen
3B +/- CX:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B -/- CX:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: neocortex |
pericyte |
Present |
Regionally restricted |
3B CX -/-
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Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.
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Specimen
3B +/- CC:
embryonic day 18.5; Olig2tm1Rth/Olig2 +
(more )
Specimen
3B +/- CC:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B -/- CC:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: corpus callosum |
pericyte |
Present |
Regionally restricted |
3B CC -/-
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Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.
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Specimen
3B +/- SVZ:
embryonic day 18.5; Olig2tm1Rth/Olig2 +
(more )
Specimen
3B +/- SVZ:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B -/- SVZ:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B +/- OB:
embryonic day 18.5; Olig2tm1Rth/Olig2 +
(more )
Specimen
3B +/- OB:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B -/- OB:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: main olfactory bulb |
pericyte |
Present |
Regionally restricted |
3B OB -/-
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Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.
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Specimen
3B +/- VFB:
embryonic day 18.5; Olig2tm1Rth/Olig2 +
(more )
Specimen
3B +/- VFB:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3B -/- VFB:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3C Olig2-/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
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Present |
Scattered |
3C left
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Scattered surviving cells were detected in the ventral forebrain.
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| TS26: forebrain |
pericyte |
Present |
Regionally restricted |
3C left
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Staining in vessel pericytes was unaffected.
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Specimen
3C Olig1/2-/-:
(close )
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: forebrain |
pericyte |
Present |
Regionally restricted |
3C right
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Only staining in vessel pericytes was unaffected. No positive cell bodies with recognizable synantocyte or bipolar morphology were seen.
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Specimen
4A +/-:
embryonic day 18.5; Olig2tm1Rth/Olig2 +
(more )
Specimen
4A +/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4A -/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Olig2tm1Rth/Olig2tm1Rth
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
pericyte |
Present |
Regionally restricted |
4A middle -/-
,
4A lower -/-
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Only staining in vessel pericytes was unaffected. No positive neural multiprocessed cells were seen.
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