Gene Expression Data
Assay Details
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Assay
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Reference:
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J:311540
Oliver BL, et al., Spatial and temporal expression of PORCN is highly dynamic in the developing mouse cochlea. Gene Expr Patterns. 2021 Sep 20;42:119214
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7547371
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Gene symbol:
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Wnt5a
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Gene name:
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wingless-type MMTV integration site family, member 5A
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Probe:
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Mm-Wnt5a (316791)
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Probe preparation:
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Antisense
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Visualized with:
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Chromogenic
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Assay notes:
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RNAscope assay.
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Results
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Specimen
2G:
embryonic day 12.5
(more  )
Specimen
2G:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cochlear duct |
Present |
Regionally restricted |
2G
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Expression is seen on the medial side and the lateral edge of the cochlea.
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Specimen
2Hi:
embryonic day 14.5
(more )
Note: base t1
Specimen
2Hi:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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base t1
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlear duct |
Present |
Regionally restricted |
2Hi
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Expression is seen in the medial sensory side and the lateral non-sensory side of the cochlea that precedes the stria vascularis.
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Specimen
2Hii:
embryonic day 14.5
(more )
Note: mid t2
Specimen
2Hii:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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mid t2
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlear duct |
Present |
Regionally restricted |
2Hii
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Expression is seen in the medial sensory side and the lateral non-sensory side of the cochlea that precedes the stria vascularis.
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Specimen
2Hiii:
embryonic day 14.5
(more )
Note: apex t3
Specimen
2Hiii:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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apex t3
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlear duct |
Present |
Regionally restricted |
2Hiii
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Expression is seen in the medial sensory side and the lateral non-sensory side of the cochlea that precedes the stria vascularis.
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Specimen
2Ii:
embryonic day 16.5
(more )
Note: base t1
Specimen
2Ii:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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base t1
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cochlear duct |
Present |
Non-Uniform |
2Ii
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Expression is seen in the inner sulcus and is downregulated in the sensory domain.
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Specimen
2Iii:
embryonic day 16.5
(more )
Note: mid t2
Specimen
2Iii:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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mid t2
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cochlear duct |
Present |
Non-Uniform |
2Iii
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Expression is seen in the inner sulcus and is downregulated in the sensory domain.
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Specimen
2Iii inset:
embryonic day 16.5
(more )
Note: mid t2. Double labeled: red - Wnt5a; blue - Myo7a (assay MGI:7547372).
Specimen
2Iii inset:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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mid t2. Double labeled: red - Wnt5a; blue - Myo7a (assay MGI:7547372).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cochlear duct |
Present |
Non-Uniform |
2Iii inset
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Expression is seen in the inner sulcus but is downregulated in the hair cells.
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Specimen
2Iiii:
embryonic day 16.5
(more )
Note: mid t3
Specimen
2Iiii:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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mid t3
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cochlear duct |
Present |
Non-Uniform |
2Iiii
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Expression is seen in the inner sulcus and is downregulated in the sensory domain.
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Specimen
2Iiv:
embryonic day 16.5
(more )
Note: apex t4
Specimen
2Iiv:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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apex t4
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cochlear duct epithelium |
Present |
Non-Uniform |
2Iiv
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Expression is seen in the inner sulcus and is downregulated in the sensory domain.
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