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Gene Expression Data
Assay Details
Assay
Reference: J:273130 Ding W, et al., Cenpj Regulates Cilia Disassembly and Neurogenesis in the Developing Mouse Cortex. J Neurosci. 2019 Mar 13;39(11):1994-2010
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7547340
Gene symbol: Tubg1
Gene name: tubulin, gamma 1
Antibody: anti-gamma-tubulin (T3559)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two gamma-tubulin antibodies were described, but the one used in this assay was not detailed. Anti-gamma-tubulin (T5326) name MGI:7547289 may have been used in this assay.
Results
Specimen 3A gamma-tubulin Cenpj+/+;CiliaGFP/+ (upper): embryonic day 15.5; Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Double labeled: red - Tubg1; green - GFP reporter.
Structure Cell Type Level Pattern Image Note
TS23: telencephalon ventricular layer radial glial cell Present Regionally restricted 3A Cenpj+/+CiliaGFP (upper)
Specimen 3A gamma-tubulin CenpjCKO;CiliaGFP/+ (upper): embryonic day 15.5; Cenpjtm1c(EUCOMM)Wtsi/Cenpjtm1c(EUCOMM)Wtsi, Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Tubg1; green - GFP reporter.
Structure Cell Type Level Pattern Image Note
TS23: telencephalon ventricular layer radial glial cell Present Regionally restricted 3A CenpjCKOCiliaGFP (upper) Expression in radial glial cells on lateral ventricle surface.
Specimen 3A gamma-tubulin Cenpj+/+;CiliaGFP/+ (lower): embryonic day 15.5; Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Double labeled: red - Tubg1; green - GFP reporter.
Structure Cell Type Level Pattern Image Note
TS23: telencephalon ventricular layer radial glial cell Present 3A Cenpj+/+CiliaGFP (lower) Expression in radial glial cells on lateral ventricle surface.
Specimen 3A gamma-tubulin CenpjCKO;CiliaGFP/+ (lower): embryonic day 15.5; Cenpjtm1c(EUCOMM)Wtsi/Cenpjtm1c(EUCOMM)Wtsi, Emx1tm1(cre)Krj/Emx1+, Gt(ROSA)26Sortm1.1(Sstr3/GFP)Bky/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Tubg1; green - GFP reporter.
Structure Cell Type Level Pattern Image Note
TS23: telencephalon ventricular layer radial glial cell Present Regionally restricted 3A CenpjCKOCiliaGFP (lower) Expression in radial glial cells on lateral ventricle surface.
Specimen 5J gamma-tubulin Cenpj+/+: postnatal adult (more )
Note: Double labeled: red - Tubg1; green - Tjp1 (assay MGI:7547338).
Structure Level Pattern Image Note
TS28: brain ventricular layer Present Regionally restricted 5J Cempj+/+ Expression in basal bodies, the majority of which were in the same direction.
Specimen 5J gamma-tubulin CenpjCKO: postnatal adult; Cenpjtm1c(EUCOMM)Wtsi/Cenpjtm1c(EUCOMM)Wtsi, Emx1tm1(cre)Krj/Emx1+ (more )
Note: Conditional mutant. Double labeled: red - Tubg1; green - Tjp1 (assay MGI:7547338).
Structure Level Pattern Image Note
TS28: brain ventricular layer Present Regionally restricted 5J CenpjCKO Basal body patches often migrated in different direction from neighboring cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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