Gene Expression Data
Assay Details
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Assay
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Reference:
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J:81418
Wang L, et al., Estrogen receptor (ER)beta knockout mice reveal a role for ERbeta in migration of cortical neurons in the developing brain. Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):703-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7547097
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Gene symbol:
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Map2
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Gene name:
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microtubule-associated protein 2
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Antibody:
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anti-MAP-2B
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
7A:
embryonic day 18.5
(more  )
Specimen
7A:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
cerebral cortex neuron |
Present |
Regionally restricted |
7A
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The neurons in the upper layers displayed in a clear laminar and radial formation.
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| TS26: neocortex |
cerebral cortex neuron |
Strong |
Non-Uniform |
7A
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Expression is stronger in layer I.
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Specimen
7B:
embryonic day 18.5; Esr2tm1Unc/Esr2 +
(more )
Specimen
7B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Esr2tm1Unc/Esr2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
cerebral cortex neuron |
Present |
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7B
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Expression in this heterozygote was similar to wild-type.
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Specimen
7C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Esr2tm1Unc/Esr2tm1Unc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
cerebral cortex neuron |
Present |
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7C
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In this mutant the cortical neurons appear smaller and disorganized with no clear radial pattern formed compared to controls.
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