Pls3 RT-PCR Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:341714 Petit F, et al., PLS3 missense variants affecting the actin-binding domains cause X-linked congenital diaphragmatic hernia and body-wall defects. Am J Hum Genet. 2023 Oct 5;110(10):1787-1803
Assay type:	RT-PCR
MGI Accession ID:	MGI:7545085
Gene symbol:	Pls3
Gene name:	plastin 3 (T-isoform)

Probe:	Pls3-pE, Pls3-pF
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Gapdh.

Results

Image: 4B - first exon

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
first exon - WT	Cultured cells: no data stored
first exon - Pls3^W99C/W99C	Cultured cells: no data stored
first exon - Pls3^{14bpdel/14bpdel}	Cultured cells: no data stored
last exon - WT	Cultured cells: no data stored
last exon - Pls3^W99C/W99C	Cultured cells: no data stored
last exon - Pls3^{14bpdel/14bpdel}	Cultured cells: no data stored
first exon - WT	E12.5	TS20: embryo	Present	Not Specified	C57BL/6NJ		Not Specified
first exon - Pls3^W99C/W99C	E12.5	TS20: embryo	Present (a)	Not Specified	C57BL/6J-Pls3^em1Bult/BultJ	Pls3^em1Bult/Pls3^em1Bult	Not Specified
first exon - Pls3^{14bpdel/14bpdel}	E12.5	TS20: embryo	Absent (b)	Not Specified	C57BL/6J-Pls3^em2Bult/BultJ	Pls3^em2Bult/Pls3^em2Bult	Not Specified
last exon - WT	Other lane(s) in blot use different probe(s)
last exon - Pls3^W99C/W99C	Other lane(s) in blot use different probe(s)
last exon - Pls3^{14bpdel/14bpdel}	Other lane(s) in blot use different probe(s)

Notes:
(a) Expression levels are comparable to those seen in wild type.
(b) Expression is almost undetectable.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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