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Gene Expression Data
Assay Details
Assay
Reference: J:186504 Tanimoto Y, et al., Prevention of premature fusion of calvarial suture in GLI-Kruppel family member 3 (Gli3)-deficient mice by removing one allele of Runt-related transcription factor 2 (Runx2). J Biol Chem. 2012 Jun 15;287(25):21429-38
Assay type: Western blot
MGI Accession ID: MGI:7543958
Gene symbol: Smad9
Gene name: SMAD family member 9
Antibody: anti-phospho-Smad1/5/9 (9511)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The antibody used recognizes the phosphorylated form of the protein. The specificity of the antibody used was not detailed; all family members have been annotated.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 55 kDa (a) Genetic BackgroundMutant Allele(s)Sex
Wt 1 E15.5 TS23: chondrocranium Present involves: C3H/HeJ * C57BL/6 Not Specified
Wt 2 E15.5 TS23: chondrocranium Present involves: C3H/HeJ * C57BL/6 Not Specified
Wt 3 E15.5 TS23: chondrocranium Present involves: C3H/HeJ * C57BL/6 Not Specified
Wt 4 E15.5 TS23: chondrocranium Present involves: C3H/HeJ * C57BL/6 Not Specified
Xt-J/Xt-J 5 E15.5 TS23: chondrocranium Present involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
Xt-J/Xt-J 6 E15.5 TS23: chondrocranium Present involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
Xt-J/Xt-J 7 E15.5 TS23: chondrocranium Present involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
Xt-J/Xt-J 8 E15.5 TS23: chondrocranium Present involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
Xt-J/Xt-J 9 E15.5 TS23: chondrocranium Present involves: C3H/HeJ * C57BL/6 Gli3Xt-J/Gli3Xt-JNot Specified
Notes:
(a) Higher band intensity was observed in the mutant compared with the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory