Probe:	JP-4 probes
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: accumbens nucleus	Strong		3A
TS28: anterior olfactory nucleus	Strong		3A
TS28: caudate-putamen	Strong		3A
TS28: cerebellar cortex	Strong		3A
TS28: hippocampal formation	Strong		3A
TS28: main olfactory bulb layer	Strong		3A
TS28: olfactory tubercle	Strong		3A
TS28: piriform cortex	Strong		3A
TS28: amygdaloid nucleus	Moderate		3A
TS28: cerebral cortex	Moderate		3A
TS28: medial habenular nucleus	Moderate		3A
TS28: paraventricular thalamic nucleus	Moderate		3A
TS28: hypothalamic nucleus	Weak		3A
TS28: inferior colliculus	Weak		3A
TS28: lateral septal nucleus	Weak		3A
TS28: substantia nigra	Weak		3A
TS28: superior colliculus	Weak		3A
TS28: zona incerta	Weak		3A
TS28: brainstem nucleus	Weak	Regionally restricted	3A	Expression is in various brain stem nuclei.

Structure	Level	Pattern	Image	Note
TS28: amygdaloid nucleus	Strong		3C
TS28: caudate-putamen	Strong		3C
TS28: hippocampal formation	Strong		3C
TS28: piriform cortex	Strong		3C

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Very strong		3E	Expression is in cell bodies.
TS28: hippocampus pyramidal cell layer	Very strong		3E	Expression is in cell bodies.
TS28: dentate gyrus molecular layer	Present	Diffuse	3E	There is diffuse hybridization evenly in dendritic fields.
TS28: hippocampus stratum lacunosum	Present	Diffuse	3E	There is diffuse hybridization evenly in dendritic fields.
TS28: hippocampus stratum oriens	Present	Diffuse	3E	There is diffuse hybridization evenly in dendritic fields.
TS28: hippocampus stratum radiatum	Present	Diffuse	3E	There is diffuse hybridization evenly in dendritic fields.
TS28: hippocampus CA3	Present	Regionally restricted	3E	Expression is absent in dendritic fields of the CA3 region of the Ammon's horn.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Strong		3G

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong		3I
TS28: cerebellum Purkinje cell layer	Weak		3I
TS28: cerebellum molecular layer	Weak		3I

Structure	Level	Pattern	Image	Note
TS23: autonomic ganglion	Strong		4A
TS23: brain mantle layer	Strong		4A
TS23: central nervous system ganglion	Strong		4A
TS23: dorsal root ganglion	Strong		4A
TS23: retina	Strong		4A
TS23: spinal cord	Strong		4A
TS23: sympathetic ganglion	Strong		4A
TS23: trigeminal ganglion	Strong		4A
TS23: brain ventricular layer	Absent		4A

Structure	Level	Pattern	Image	Note
TS28: accumbens nucleus	Strong
TS28: anterior olfactory nucleus	Strong
TS28: caudate-putamen	Strong
TS28: cerebellum granule cell layer	Strong
TS28: hippocampus granule cell layer	Strong
TS28: hippocampus pyramidal cell layer	Strong
TS28: main olfactory bulb	Strong
TS28: amygdala	Moderate
TS28: cerebral cortex	Moderate
TS28: medial habenular nucleus	Moderate
TS28: paraventricular thalamic nucleus	Moderate
TS28: thalamic nucleus	Moderate	Regionally restricted		Expression is in the paraventricular thalamic nucleus and is absent in the ventrolateral thalamic nucleus, ventroposterior thalamic nucleus, and posterior thalamic nucleus.
TS28: cerebellum Purkinje cell layer	Weak
TS28: facial nucleus	Weak
TS28: hypothalamus	Weak
TS28: inferior colliculus	Weak
TS28: lateral septal nucleus	Weak
TS28: substantia nigra	Weak
TS28: superior colliculus	Weak
TS28: zona incerta	Weak
TS28: deep cerebellar nucleus	Absent
TS28: posterior thalamic nucleus	Absent
TS28: spinal cord gray matter	Absent