Htr2aem3(cre)Blr
Endonuclease-mediated Allele Detail
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| Symbol: |
Htr2aem3(cre)Blr |
| Name: |
5-hydroxytryptamine (serotonin) receptor 2A; endonuclease-mediated mutation 3, Bryan L Roth |
| MGI ID: |
MGI:7543067 |
| Synonyms: |
Htr2aA242S-EGFP-Cre, Htr2a-A242S-EGFP-CT-IRES-Cre, Htr2aem3(EGFP, cre)Blr |
| Gene: |
Htr2a Location: Chr14:74878314-74944299 bp, + strand Genetic Position: Chr14, 39.37 cM
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| Alliance: |
Htr2aem3(cre)Blr page
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| Allele Type: |
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Endonuclease-mediated (Humanized sequence, Recombinase, Reporter) |
| Mutations: |
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Insertion, Nucleotide substitutions
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Htr2aem3(cre)Blr expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Htr2a (MGI:109521) |
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Mutation details: A cDNA encoding EGFP (flanked on both ends by a serine- and glycine- rich linker) was inserted into the C-terminus (CT) of the mouse Htr2a gene after residue 452 (encoded by exon 3; chr14:74706337 in the GRCm38/mm10 genome assembly) and an IRES-Cre cassette was placed between the stop codon and the 3' UTR (chr14:74706397). A single humanizing A242S (nucleotide: GCA to TCC) mutation was also introduced to exon 3 of the mouse Htr2a gene. The allele was created using CRISPR/cas9 methodologies in C57BL/6J embryos.
(J:341620)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Htr2a
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Htr2a Mutation: |
45 strains or lines available
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| Original: |
J:341620 Chiu YT, et al., A suite of engineered mice for interrogating psychedelic drug actions. bioRxiv. 2023 Sep 26; |
| All: |
1 reference(s) |
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Analysis Tools
