Gene Expression Data
Assay Details
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Assay
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Reference:
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J:341326
Nag P, et al., Tmem2 Deficiency Leads to Enamel Hypoplasia and Soft Enamel in Mouse. J Dent Res. 2023 Sep;102(10):1162-1171
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7539811
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Gene symbol:
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Cemip2
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Gene name:
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cell migration inducing hyaluronidase 2
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Reporter:
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FLAG
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Antibody:
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anti-FLAG (7425)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
1B Pre-ameloblast:
postnatal day 14; Cemip2em1Osb/Cemip2 +
(more  )
Note: Double labeled: green - Cemip2; red - hyaluronic acid binding protein.
Specimen
1B Pre-ameloblast:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Cemip2em1Osb/Cemip2+
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Cemip2; red - hyaluronic acid binding protein.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: incisor enamel organ |
ameloblast |
Present |
Regionally restricted |
1B PAB TMEM2/HA
,
1B PAB TMEM2/DAPI
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Expression in presecretory ameloblasts.
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| TS28: incisor |
odontoblast |
Present |
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1B PAB TMEM2/DAPI
,
1B PAB TMEM2/HA
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| TS28: stratum intermedium |
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Present |
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1B PAB TMEM2/DAPI
,
1B PAB TMEM2/HA
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Specimen
1B Secondary-ameloblast:
postnatal day 14; Cemip2em1Osb/Cemip2 +
(more )
Note: Double labeled: green - Cemip2; red - hyaluronic acid binding protein.
Specimen
1B Secondary-ameloblast:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Cemip2em1Osb/Cemip2+
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Cemip2; red - hyaluronic acid binding protein.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: incisor enamel organ |
ameloblast |
Present |
Regionally restricted |
1B SAB TMEM2/HA (upper)
,
1B SAB TMEM2/HA (lower)
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1B SAB TMEM2/DAPI (lower)
,
1B SAB TMEM2/DAPI (upper)
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Expression in secretory ameloblasts, localized at apical and basal ends of ameloblasts.
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| TS28: incisor |
odontoblast |
Present |
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1B SAB TMEM2/HA (lower)
,
1B SAB TMEM2/DAPI (lower)
,
1B SAB TMEM2/HA (upper)
,
1B SAB TMEM2/DAPI (upper)
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| TS28: stratum intermedium |
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Present |
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1B SAB TMEM2/HA (lower)
,
1B SAB TMEM2/HA (upper)
,
1B SAB TMEM2/DAPI (lower)
,
1B SAB TMEM2/DAPI (upper)
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Specimen
1B Mature-ameloblast:
postnatal day 14; Cemip2em1Osb/Cemip2 +
(more )
Note: Double labeled: green - Cemip2; red - hyaluronic acid binding protein.
Specimen
1B Mature-ameloblast:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Cemip2em1Osb/Cemip2+
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Cemip2; red - hyaluronic acid binding protein.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: incisor enamel organ |
ameloblast |
Present |
Regionally restricted |
1B MAB TMEM2/HA
,
1B MAB TMEM2/DAPI
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Expression in maturation stages of ameloblasts.
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| TS28: incisor |
odontoblast |
Present |
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1B MAB TMEM2/DAPI
,
1B MAB TMEM2/HA
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| TS28: stratum intermedium |
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Present |
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1B MAB TMEM2/HA
,
1B MAB TMEM2/DAPI
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Analysis Tools