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Gene Expression Data
Assay Details
Assay
Reference: J:337132 Hiremath C, et al., Rap1 regulates lumen continuity via Afadin in renal epithelia. Dev Biol. 2023 Jun 3;501:20-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7539440
Gene symbol: Muc1
Gene name: mucin 1, transmembrane
Antibody: MUC1 Antibody (MH1 (CT2)) (MA5-11202)
Detection system: Secondary antibody
Results
Specimen 4A control left: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Note: 3D projections from a confocal z-stack.
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present 4A control left Expressed at the apical surface.
TS22: nephron Present Regionally restricted 4A control left Expressed in the distal nephron.
Specimen 4A control right: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Note: 3D projections from the central images.
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present 4A control right Expressed at the apical surface.
TS22: nephron Present Regionally restricted 4A control right Expressed in the distal nephron.
Specimen 4A cKO left: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. 3D projections from a confocal z-stack.
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present 4A cKO left Similar as in the controls, expressed at the apical surface.
TS22: nephron Present Regionally restricted 4A cKO left Expressed in the distal nephron, similar as in the controls.
Specimen 4A cKO right: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. 3D projections from the central images.
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present 4A cKO right Similar as in the controls, expressed at the apical surface.
TS22: nephron Present Regionally restricted 4A cKO right Expressed in the distal nephron, similar as in the controls.
Specimen 4B control: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz (more )
Note: Double labeled: white - Muc1; purple - Cdh1 (assay MGI:7539441).
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present 4B control right , 4B control left Expressed at the apical surface.
Specimen 4B cKO: embryonic day 14.5; Rap1atm1Morz/Rap1atm1Morz, Rap1btm1Morz/Rap1btm1Morz, Tg(Hoxb7-cre)13Amc/0 (more )
Note: Conditional mutant. Double labeled: white - Muc1; purple - Cdh1 (assay MGI:7539441).
Structure Cell Type Level Pattern Image Note
TS22: collecting duct kidney collecting duct epithelial cell Present 4B cKO right , 4B cKO left Similar as in the controls, expressed at the apical surface.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory