Antibody:	anti-xCT
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Present		3a
TS28: retina ganglion cell layer	Present		3a
TS28: retina inner nuclear layer	Present		3a
TS28: retina inner plexiform layer	Present		3a
TS28: retina outer nuclear layer	Present		3a
TS28: retina outer plexiform layer	Present		3a

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Present		3b
TS28: retina ganglion cell layer	Present		3b
TS28: retina inner nuclear layer	Present		3b
TS28: retina inner plexiform layer	Present		3b
TS28: retina outer nuclear layer	Present		3b
TS28: retina outer plexiform layer	Present		3b

Structure	Level	Pattern	Image	Note
TS28: ciliary epithelium	Present	Regionally restricted	4a	Expression is in the the outer non-pigmented epithelium and is absent in the inner pigmented epithelial cell layer.

Structure	Level	Pattern	Image	Note
TS28: ciliary epithelium	Present		4b	Expression in this mutant is the same as in wild-type.

Structure	Level	Pattern	Image	Note
TS28: iris	Absent		5a

Structure	Level	Pattern	Image	Note
TS28: iris	Absent		5b

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	6a	Expression is in the peripheral and central regions of the cornea.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	6b	Expression is in the peripheral and central regions of the cornea.
TS28: cornea endothelium	Absent		6b
TS28: cornea stroma	Absent		6b

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	6c	Expression is in the peripheral and central regions of the cornea.
TS28: cornea endothelium	Absent		6c
TS28: cornea stroma	Absent		6c

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Absent		6d	The authors state that the faint staining as a thin line outlining the outermost superficial layer of the epithelium is likely an artefact caused by the antibody 'sticking' to the edges of the section, since this staining was also seen in wild-type corneal sections.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Absent		6e	The authors state that the faint staining as a thin line outlining the outermost superficial layer of the epithelium is likely an artefact caused by the antibody 'sticking' to the edges of the section, since this staining was also seen in wild-type corneal sections.

Structure	Level	Pattern	Image	Note
TS28: lens	Present	Regionally restricted	7b	Expression is in the outer cortex localized to the broad sides of the membranes of the outer cortical fiber cells.
TS28: lens fiber	Present	Regionally restricted	7b	Expression is in the outer cortex localized to the broad sides of the membranes of the outer cortical fiber cells.
TS28: lens epithelium	Present		7b	There is faint cytoplasmic labeling and along the apical-apical interface of the epithelium-fiber cell border.

Structure	Level	Pattern	Image	Note
TS28: lens fiber	Present	Single cells	7c	There is sporadic membranous labeling in the inner cortical fiber cells.

Structure	Level	Pattern	Image	Note
TS28: lens	Present	Regionally restricted	7d	Expression is absent in the lens center or core region.

Structure	Level	Pattern	Image	Note
TS28: lens	Absent		7e

Structure	Level	Pattern	Image	Note
TS28: lens	Absent		7f

Structure	Level	Pattern	Image	Note
TS28: lens	Absent		7g

Structure	Level	Pattern	Image	Note
TS24: lens anterior epithelium	Strong		8c , 8b , 8d , 8a
TS24: lens equatorial epithelium	Strong		8c , 8b , 8d , 8a
TS24: lens fiber	Strong		8a , 8b , 8d , 8c	Expression is in primary and secondary fiber cells.

Structure	Level	Pattern	Image	Note
TS27: lens anterior epithelium	Strong		8h , 8f , 8g , 8e
TS27: lens equatorial epithelium	Strong		8h , 8f , 8g , 8e
TS27: lens fiber	Present	Non-Uniform	8g , 8f , 8h , 8e	Expression is weaker in the cortical fiber cells in the anterior pole and equator and was virtually absent in the center.

Structure	Level	Pattern	Image	Note
TS28: lens fiber	Present	Regionally restricted	8j , 8i , 8l , 8k	Expression is absent in the lens core.